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. 1987 Sep 15;246(3):619-23.
doi: 10.1042/bj2460619.

Phorbol-ester-induced alterations of free calcium ion transients in single rat hepatocytes

N M Woods  1 , K S Cuthbertson, P H Cobbold
Affiliations

Phorbol-ester-induced alterations of free calcium ion transients in single rat hepatocytes

N M Woods et al. Biochem J. .

Abstract

The effect of the phorbol esters phorbol 12-myristate 13-acetate (TPA) and phorbol 12,13-dibutyrate (PDB) on changes in free Ca2+ concentration ([Ca2+]i) in single rat hepatocytes, microinjected with the photoprotein aequorin, were investigated. [Arg8]vasopressin and phenylephrine induced a series of repetitive [Ca2+]i transients. Phorbol esters inhibited the alpha 1-adrenoceptor-induced response; sub-nanomolar concentrations decreased the transient frequency, and higher concentrations abolished the transients. The inhibitory effect of PDB was readily reversible. Phorbol esters were less effective in decreasing the frequency of [Arg8]-vasopressin-induced transients, and the inhibition could be overcome by high [Arg8]vasopressin concentrations.

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References

    1. Nature. 1986 Feb 13-19;319(6054):600-2 - PubMed
    1. Basic Res Cardiol. 1985;80 Suppl 2:155-8 - PubMed
    1. Biochem J. 1986 Sep 15;238(3):737-43 - PubMed
    1. Cell Calcium. 1987 Feb;8(1):79-100 - PubMed
    1. Biochem Biophys Res Commun. 1979 Dec 28;91(4):1218-24 - PubMed

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