Evaluation of the interaction between insecticide resistance-associated genes and malaria transmission in Anopheles gambiae sensu lato in central Côte d'Ivoire

Abstract

Background: There is evidence that the knockdown resistance gene (Kdr) L1014F and acetylcholinesterase-1 gene (Ace-1^R) G119S mutations involved in pyrethroid and carbamate resistance in Anopheles gambiae influence malaria transmission in sub-Saharan Africa. This is likely due to changes in the behaviour, life history and vector competence and capacity of An. gambiae. In the present study, performed as part of a two-arm cluster randomized controlled trial evaluating the impact of household screening plus a novel insecticide delivery system (In2Care Eave Tubes), we investigated the distribution of insecticide target site mutations and their association with infection status in wild An. gambiae sensu lato (s.l.) populations.

Methods: Mosquitoes were captured in 40 villages around Bouaké by human landing catch from May 2017 to April 2019. Randomly selected samples of An. gambiae s.l. that were infected or not infected with Plasmodium sp. were identified to species and then genotyped for Kdr L1014F and Ace-1^R G119S mutations using quantitative polymerase chain reaction assays. The frequencies of the two alleles were compared between Anopheles coluzzii and Anopheles gambiae and then between infected and uninfected groups for each species.

Results: The presence of An. gambiae (49%) and An. coluzzii (51%) was confirmed in Bouaké. Individuals of both species infected with Plasmodium parasites were found. Over the study period, the average frequency of the Kdr L1014F and Ace-1^R G119S mutations did not vary significantly between study arms. However, the frequencies of the Kdr L1014F and Ace-1^R G119S resistance alleles were significantly higher in An. gambiae than in An. coluzzii [odds ratio (95% confidence interval): 59.64 (30.81-131.63) for Kdr, and 2.79 (2.17-3.60) for Ace-1^R]. For both species, there were no significant differences in Kdr L1014F or Ace-1^R G119S genotypic and allelic frequency distributions between infected and uninfected specimens (P > 0.05).

Conclusions: Either alone or in combination, Kdr L1014F and Ace-1^R G119S showed no significant association with Plasmodium infection in wild An. gambiae and An. coluzzii, demonstrating the similar competence of these species for Plasmodium transmission in Bouaké. Additional factors including behavioural and environmental ones that influence vector competence in natural populations, and those other than allele measurements (metabolic resistance factors) that contribute to resistance, should be considered when establishing the existence of a link between insecticide resistance and vector competence.

Keywords: Acetylcholinesterase-1 gene G119S mutation; Anopheles coluzzii; Anopheles gambiae; Côte d’Ivoire; Knockdown resistance gene L1014F mutation; Malaria transmission; Resistance.

Fig. 1

Anopheles gambiae sensu lato distribution by infection status. Error bars represent 95% confidence intervals (CIs). SET Screening plus In2Care Eave Tubes

Fig. 2

Frequencies of Kdr and Ace-1^R genotypic combinations between infected and uninfected groups in each study arm. Error bars represent 95% CIs. For all combined genotypes, the first two alleles refer to Kdr genotypes and the last two refer to Ace-1^R genotypes. RR Resistant homozygous genotype, RS heterozygous genotype, SS susceptible homozygous genotype; for other abbreviations, see Fig. 1