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. 2022 Feb;187(1):129-131.
doi: 10.1007/s11046-021-00602-9. Epub 2021 Nov 20.

Evaluation of OIDx Histoplasma Urinary Antigen EIA

Diego H Cáceres  1   2 , Beatriz L Gómez  3 , Ángela M Tobón  4   5 , Tom M Chiller  6 , Mark D Lindsley  6
Affiliations

Evaluation of OIDx Histoplasma Urinary Antigen EIA

Diego H Cáceres et al. Mycopathologia. 2022 Feb.

Abstract

A sandwich enzyme immunoassay (EIA) for the detection of Histoplasma antigens (Ag) in urine, developed by Optimum Imaging Diagnostics (OIDx) was evaluated. A verification using a standardized reference panel of urine samples found sensitivity of 92%, specificity of 32% and accuracy of 51%. In this study, the OIDx Histoplasma urinary Ag EIA displayed high sensitivity, however, in non-histoplasmosis cases this EIA displayed false-positive results in 68% of specimens tested.

Keywords: Antigen; Enzyme immunoassay; Histoplasma; Histoplasmosis.

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References

    1. Wheat LJ, Connolly-Stringfield P, Kohler RB, Frame PT, Gupta MR. Histoplasma capsulatum polysaccharide antigen detection in diagnosis and management of disseminated histoplasmosis in patients with acquired immunodeficiency syndrome. Am J Med. 1989;87(4):396–400. - DOI
    1. Swartzentruber S, LeMonte A, Witt J, Fuller D, Davis T, Hage C, et al. Improved detection of Histoplasma antigenemia following dissociation of immune complexes. Clin Vaccine Immunol. 2009;16(3):320–2. - DOI
    1. Connolly PA, Durkin MM, Lemonte AM, Hackett EJ, Wheat LJ. Detection of Histoplasma antigen by a quantitative enzyme immunoassay. Clin Vaccine Immunol. 2007;14(12):1587–91. - DOI
    1. Caceres DH, Samayoa BE, Medina NG, Tobon AM, Guzman BJ, Mercado D, et al. Multicenter validation of commercial antigenuria reagents to diagnose progressive disseminated histoplasmosis in people living with HIV/AIDS in two Latin American countries. J Clin Microbiol. 2018;56(6).
    1. Martínez-Gamboa A, Niembro-Ortega MD, Torres-González P, Santiago-Cruz J, Velázquez-Zavala NG, Rangel-Cordero A, et al. Diagnostic accuracy of antigen detection in urine and molecular assays testing in different clinical samples for the diagnosis of progressive disseminated histoplasmosis in patients living with HIV/AIDS: a prospective multicenter study in Mexico. PLoS Negl Trop Dis. 2021;15(3):e0009215. - DOI

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