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. 2021 Nov 12:2021:3037993.
doi: 10.1155/2021/3037993. eCollection 2021.

Nuclear Morphological Characteristics in Breast Cancer: Correlation with Hormone Receptor and Human Epidermal Growth Factor Receptor 2

Jiayu Li  1 , Yehan Zhou  1 , Yunzhu Li  1 , Yang Liu  1
Affiliations

Nuclear Morphological Characteristics in Breast Cancer: Correlation with Hormone Receptor and Human Epidermal Growth Factor Receptor 2

Jiayu Li et al. Anal Cell Pathol (Amst). .

Abstract

Background: Hormone receptor (HR) and human epidermal growth factor receptor 2 (HER2) are the common diagnostic/prognostic markers in breast cancer. Few articles have recently reported the correlation between cytology and molecular subtypes. We combined nuclear morphological characteristics with HR and HER2 status to observe the relationship and provide ideas for machine learning.

Methods: We reanalyzed fine-needle aspiration cytology samples and core-needle puncture histological specimens from 142 patients with invasive breast cancer between March 2019 and December 2019, and the findings were compared with the two groups (HR+/HER2- and HR-/HER2+) following nuclear cytomorphological features: nuclear/cytoplasmic ratio, difference of nuclear size, nuclear pleomorphism, chromatin feature, nuclear membrane and nucleoli, and Nottingham grading.

Results: Two groups were significantly associated with the difference of nuclear size, nuclear pleomorphism, and nucleoli (P < 0.001) and consistent with histological grading (P < 0.001). Moreover, nucleolar characteristics of size and number had obviously statistical significance (P < 0.001). Multiple micro-nucleoli were frequently seen in the HR+/HER2- group compared with the HR-/HER2+ group which mostly were observed centered medium-large nucleoli. We described four interesting nuclear morphologies in the experiment.

Conclusions: There were significant differences in nuclear characteristics between two groups. HR and HER2 status not only might be predicted in cytological samples, but some specific nuclear morphological features might have potential value to help us understand molecular function and predict more information.

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Conflict of interest statement

The authors state that there are no conflicts of interest to disclose.

Figures

Figure 1
Figure 1
Patterns of four special nuclear morphologies.
Figure 2
Figure 2
(a) Ground glass-like nuclei and (b) haze-like nuclei: nuclear membrane, light and homogeneous chromatin, micronucleoli (FNAC, ×20).
Figure 3
Figure 3
(a) Solidified-like nuclei and (b) flocculated-like nuclei: prominent nucleoli and halos around the nucleoli or heterogeneous chromatin (FNAC, ×20).
Figure 4
Figure 4
(a, b) Histological characteristics of HR+/HER2- group (CNB, ×20 and ×40).
Figure 5
Figure 5
(a, b) Histological characteristics of HR-/HER2+ group (CNB, ×20 and ×40).
See this image and copyright information in PMC

References

    1. Sørlie T., Tibshirani R., Parker J., et al. Repeated observation of breast tumor subtypes in independent gene expression data sets. Proceedings of the National Academy of Sciences of the United States of America . 2003;100(14):8418–8423. doi: 10.1073/pnas.0932692100. - DOI - PMC - PubMed
    1. Prossnitz E. R., Barton M. Estrogen biology: new insights into GPER function and clinical opportunities. Molecular and Cellular Endocrinology . 2014;389(1-2):71–83. doi: 10.1016/j.mce.2014.02.002. - DOI - PMC - PubMed
    1. Forster C., Makela S., Warri A., et al. Involvement of estrogen receptor beta in terminal differentiation of mammary gland epithelium. Proceedings of the National Academy of Sciences of the United States of America . 2002;99(24):15578–15583. doi: 10.1073/pnas.192561299. - DOI - PMC - PubMed
    1. Klapper L. N., Glathe S., Vaisman N., et al. The ErbB-2/HER2 oncoprotein of human carcinomas may function solely as a shared coreceptor for multiple stroma-derived growth factors. Proceedings of the National Academy of Sciences of the United States of America . 1999;96(9):4995–5000. doi: 10.1073/pnas.96.9.4995. - DOI - PMC - PubMed
    1. Bauer K. R., Brown M., Cress R. D., Parise C. A., Caggiano V. Descriptive analysis of estrogen receptor (ER)-negative, progesterone receptor (PR)-negative, and HER2-negative invasive breast cancer, the so-called triple-negative phenotype. Cancer-Am Cancer Soc. . 2007;109(9):1721–1728. doi: 10.1002/cncr.22618. - DOI - PubMed