Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Nov;1(11):e294.
doi: 10.1002/cpz1.294.

Characterization of Campylobacter jejuni-Neutrophil Interactions

Sean M Callahan  1 , Trevor J Hancock  1 , Jeremiah G Johnson  1
Affiliations

Characterization of Campylobacter jejuni-Neutrophil Interactions

Sean M Callahan et al. Curr Protoc. 2021 Nov.

Abstract

Campylobacter jejuni is the leading cause of bacterial-derived gastroenteritis worldwide, infecting 96 million individuals annually. During infection, inflammation and tissue pathology occur in the lower gastrointestinal tract, including the recruitment of leukocytes. Neutrophils are the most abundant leukocyte in humans, and recruitment is associated with bacterial infections and the development of various inflammatory disorders, including inflammatory bowel disease. Neutrophils possess three main antibacterial functions: phagocytosis and degradation of microbes, degranulation to release antimicrobial proteins, and extrusion of neutrophil extracellular traps (NETs). Because neutrophils are recruited to the site of C. jejuni infection and they are associated with damaging inflammation in other diseases, it is imperative to understand the immunopathology that occurs during C. jejuni infection and thoroughly study the neutrophil response to the pathogen. Detailed protocols for human and ferret neutrophil isolations, neutrophil gentamicin protection assay, neutrophil activation flow cytometry assay, NET induction and quantification, and neutrophil western blot analysis are included in this article. © 2021 Wiley Periodicals LLC. Basic Protocol 1: Isolation of human and ferret neutrophils Basic Protocol 2: Neutrophil gentamicin protection assay Basic Protocol 3: Neutrophil activation flow cytometry analyses Basic Protocol 4: Neutrophil extracellular trap induction and quantification Basic Protocol 5: Western blot detection of neutrophil-derived antimicrobial proteins.

Keywords: Campylobacter; host-microbe interactions; infection; neutrophil; pathogenesis.

PubMed Disclaimer

References

Literature Cited

    1. Burn, G. L., Foti, A., Marsman, G., Patel, D. F., & Zychlinsky, A. (2021). The neutrophil. Immunity, 54(7), 1377-1391. doi: 10.1016/j.immuni.2021.06.006.
    1. Callahan, S., Doster, R. S., Jackson, J. W., Kelley, B. R., Gaddy, J. A., & Johnson, J. G. (2020). Induction of neutrophil extracellular traps by Campylobacter jejuni. Cellular Microbiology, 22(8), e13210. doi: 10.1111/cmi.13210.
    1. Fox, J. G., Ackerman, J. I., Taylor, N., Claps, M., & Murphy, J. C. (1987). Campylobacter jejuni infection in the ferret: An animal model of human campylobacteriosis. American Journal of Veterinary Research, 48(1), 85-90.
    1. Gallagher, S. R. (2012). One-dimensional SDS gel electrophoresis of proteins. Current Protocols in Molecular Biology, 97, 10.2A.1-10.2A.44. doi: 10.1002/0471142727.mb1002as97.
    1. He, Y., Yang, F.-Y., & Sun, E.-W. (2018). Neutrophil extracellular traps in autoimmune diseases. Chinese Medical Journal, 131(13), 1513-1519. doi: 10.4103/0366-6999.235122.

Grants and funding

LinkOut - more resources