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. 2021 Nov 23;14(1):278.
doi: 10.1186/s12920-021-01125-4.

Transcriptome sequencing identified the ceRNA network associated with recurrent spontaneous abortion

Yong Huang #  1 , Jiayuan Hao #  1 , Yuan Liao  1 , Lihua Zhou  1 , Kaiju Wang  1 , Hui Zou  1 , Ying Hu  1 , Juan Li  2
Affiliations

Transcriptome sequencing identified the ceRNA network associated with recurrent spontaneous abortion

Yong Huang et al. BMC Med Genomics. .

Abstract

Background: Recurrent spontaneous abortion (RSA) is one of the common complication of pregnancy, bringing heavy burden to the patients and their families. The study aimed to explore the lncRNA-miRNA-mRNA network associated with recurrent spontaneous abortion.

Methods: By transcriptome sequencing, we detected differences in lncRNA, miRNA and mRNA expression in villus tissue samples collected from 3 patients with RSA and 3 normal abortion patients. Differentially expressed lncRNAs, miRNAs and genes (DELs, DEMs and DEGs, respectively) were identified, and Geno Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were used to determine the functions of DELs and DEGs, which were analysed by Fisher's test. We also observed the regulatory relationships between miRNA-mRNA and lncRNA-miRNA by Cytoscape 3.6.1.

Results: The results showed that 1008 DELs (523 upregulated and 485 downregulated), 475 DEGs (201 upregulated and 274 downregulated) and 37 DEMs (15 upregulated and 22 downregulated) were identified. And we also constructed a novel lncRNA-related ceRNA network containing 31 lncRNAs, 1 miRNA (hsa-miR-210-5p) and 3 genes (NTNG2, GRIA1 and AQP1).

Conclusions: lncRNA-related ceRNA network containing 31 lncRNAs, 1 miRNA (hsa-miR-210-5p) and 3 mRNAs (NTNG2, GRIA1 and AQP1) was constructed. The results may provide a basic theory for elucidating the mechanism underlying RSA.

Keywords: Recurrent spontaneous abortion; Transcriptome sequencing; ceRNA network; lncRNA.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Heatmap analysis of DEL, DEM and DEGs. DEL: Differentially expressed lncRNAs; DEMs: Differentially expressed miRNAs; DEGs: Differentially expressed genes
Fig. 2
Fig. 2
GO enrichment items and KEGG pathway analysis of DELs. a Showed that the top 10 enriched GO pathways of DELs were sorted by significance in biological process (BP), cellular component (CC) and molecular function (MF), respectively. b Showed the top 10 enriched KEGG pathways of DELs
Fig. 3
Fig. 3
GO enrichment items and KEGG pathway analysis of DEGs. a Showed that the top 10 enriched GO pathways of DEGs were sorted by significance in biological process (BP), cellular component (CC) and molecular function (MF), respectively. b Showed the top 10 enriched KEGG pathways of DEGs
Fig. 4
Fig. 4
The interactions between lncRNA-miRNA and miRNA-genes were determined, respectively. a Showed the relationship between downregulated lncRNAs and upregulated miRNAs. b Listed the interactions between hsa-miR-210-5p and 3 genes
Fig. 5
Fig. 5
The ceRNA network was constructed between DELs, DEMs and DEGs
See this image and copyright information in PMC

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