Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022:2411:95-104.
doi: 10.1007/978-1-0716-1888-2_5.

Use of a Ferret Model to Test Efficacy and Immunogenicity of Live Attenuated Mycobacterium avium Subspecies paratuberculosis Vaccines

John P Bannantine  1 , Tuhina Gupta  2 , Denise K Zinniel  3 , Ahmed Hikal  2 , Frederick D Quinn  2 , Raul G Barletta  3
Affiliations

Use of a Ferret Model to Test Efficacy and Immunogenicity of Live Attenuated Mycobacterium avium Subspecies paratuberculosis Vaccines

John P Bannantine et al. Methods Mol Biol. 2022.

Abstract

Native hosts for the bacterial agent that causes Johne's disease are ruminants, which include cattle, sheep and goats among others. These large animals are often too costly to be used in testing experimental vaccines. In this chapter, we provide detailed methods to use an inexpensive and more manageable animal host, the ferret, to test efficacy and immunogenicity of live-attenuated Mycobacterium avium subspecies paratuberculosis (MAP) mutant strains prior to consideration as vaccine candidates.

Keywords: Animal model; Ferrets; Johne’s disease; Mycobacterium; Paratuberculosis.

PubMed Disclaimer

References

    1. Hines ME 2nd, Stabel JR, Sweeney RW, Griffin F, Talaat AM, Bakker D et al (2007) Experimental challenge models for Johne’s disease: a review and proposed international guidelines. Vet Microbiol 122(3–4):197–222 - DOI
    1. Begg DJ, Whittington RJ (2008) Experimental animal infection models for Johne’s disease, an infectious enteropathy caused by Mycobacterium avium subsp. paratuberculosis. Vet J 176(2):129–145. https://doi.org/10.1016/j.tvjl.2007.02.022 - DOI - PubMed
    1. Stabel JR, Palmer MV, Harris B, Plattner B, Hostetter J, Robbe-Austerman S (2009) Pathogenesis of Mycobacterium avium subsp. paratuberculosis in neonatal calves after oral or intraperitoneal experimental infection. Vet Microbiol 136(3–4):306–313. https://doi.org/10.1016/j.vetmic.2008.11.025 - DOI - PubMed
    1. Stabel JR, Robbe-Austerman S (2011) Early immune markers associated with Mycobacterium avium subsp. paratuberculosis infection in a neonatal calf model. Clin Vaccine Immunol 18(3):393–405. https://doi.org/10.1128/CVI.00359-10 - DOI - PubMed - PMC
    1. Maher JA, DeStefano J (2004) The ferret: an animal model to study influenza virus. Lab Anim (NY) 33(9):50–53. https://doi.org/10.1038/laban1004-50 - DOI

Publication types

LinkOut - more resources