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. 2021 Nov 24;10(47):e0096521.
doi: 10.1128/MRA.00965-21. Epub 2021 Nov 24.

Complete Genome Sequence of Salmonella enterica Bacteriophage PRF-SP1

Prasanna Mutusamy  1 , Sasireigga Jaya Jothi  1 , Su Yin Lee  1   2 , Bent Petersen  1   3 , Thomas Sicheritz-Ponten  1   3 , Martha R J Clokie  4 , Stella Loke  5 , Andrew Millard  4 , Sivachandran Parimannan  1   5 , Heera Rajandas  1   5
Affiliations

Complete Genome Sequence of Salmonella enterica Bacteriophage PRF-SP1

Prasanna Mutusamy et al. Microbiol Resour Announc. .

Abstract

We characterized the complete genome sequence of the lytic Salmonella enterica bacteriophage PRF-SP1, isolated from Penang National Park, a conserved rainforest in northern Malaysia. The novel phage species from the Autographiviridae family has a 39,966-bp double-stranded DNA (dsDNA) genome containing 49 protein-encoding genes and shares 90.96% similarity with Escherichia phage DY1.

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Figures

FIG 1
FIG 1
(a) Transmission electron microscopy image of phage PRF-SP1. The scale bar represents 100 nm. The size estimate of the virion was based on an average of 10 particles. (b) Phylogenetic analysis of Salmonella phage PRF-SP1. The top hits from BLASTn were used to identify related phages. The phylogeny was based on aligned core genes identified using Roary, with trees built using the neighbor-joining method with FastTree v2.1 (15). The bootstrap value was replicated 1,000 times (parameters, -boot 1000 -noml -nt), and the FastTree output was then visualized using MEGA v11.0.8. All phages identified were classified into the family Autographiviridae, subfamily Studiervirinae, and genus Kayfunavirus. Salmonella phage PRF-SP1 is highlighted in green.
See this image and copyright information in PMC

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