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. 2021 Nov 17:20:11769351211056295.
doi: 10.1177/11769351211056295. eCollection 2021.

Effects of melatonin on the Bisphenol-A- induced cytotoxicity and genetic toxicity in colon cancer cell lines, normal gingival cell lines, and bone marrow stem cell lines

Rouya Ebrahimi  1 , Mohammad Shokrzadeh  1 , Nasrin Ghassemi Barghi  1
Affiliations

Effects of melatonin on the Bisphenol-A- induced cytotoxicity and genetic toxicity in colon cancer cell lines, normal gingival cell lines, and bone marrow stem cell lines

Rouya Ebrahimi et al. Cancer Inform. .

Abstract

Bisphenol-A (BPA) is a synthetic chemical that has widely been used in the production of polycarbonate plastic and epoxy resins in the manufacture of consumer products. The most common path of human exposure to BPA is by oral intake that involves genotoxicity, oxidative stress, endocrine disruption, mutagenicity, and carcinogenicity in both in vitro and in vivo models. Melatonin is known as a free-radical scavenger and a powerful antioxidant agent. This study aimed to investigate the effects of melatonin on viability and genetic disorders of normal Human Gingival Fibroblasts (HGF), colon cancer (MKN45), and bone marrow stem cell (MSC) lines exposed to BPA. For this purpose, MTT and Comet assays were performed to evaluate the cytotoxicity and genotoxicity properties of BPA and the role of melatonin. The results showed that BPA exposure resulted in increased oxidative stress parameters including MDA and ROS, and decreased GSH content. The current study demonstrated the cytotoxicity and genotoxicity effects of BPA and the protective role of melatonin in preventing cytotoxicity and DNA damage are induced by BPA.

Keywords: Bisphenol A; Comet; Cytotoxicity; Genotoxicity; MTT; Melatonin.

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Conflict of interest statement

Declaration Of Conflicting Interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Figures

Figure 1.
Figure 1.
The cytotoxicity of BPA and melatonin effect on BPA-induced cytotoxicity on stem cells, HGF, and MKN45 cell lines. ψψψψP < .0001: significant to the control group.
Figure 2.
Figure 2.
The genotoxicity of BPA and melatonin effect on BPA-induced genotoxicity on HGF, stem cells, and MKN45 cell lines. ψψψψP < .0001: significant to the control group. ψψψP < .001: significant to control group. ψψP < .01: significant to the control group.
Figure 3.
Figure 3.
The effects of BPA and melatonin on ROS content in the stem cell line, HGF, and MKN45 cell lines. ψψψψP < .0001: significant to the control group. ψψP < .01: significant to the control group.
Figure 4.
Figure 4.
The effects of BPA and melatonin on GSH content in the stem cell line, HGF, and MKN45 cell lines. ψψψψP < .0001: significant to the control group. ψP < .05: significant to the control group.
Figure 5.
Figure 5.
The effects of BPA and melatonin on MDA levels in the stem cell, MKN45, and HGF cell lines. ψψψψP < .0001: significant to the control group.
See this image and copyright information in PMC

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