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. 2022 Oct;26(5):2535-2548.
doi: 10.1007/s11030-021-10351-y. Epub 2021 Nov 25.

Detection of a target protein (GroEl2) in Mycobacterium tuberculosis using a derivative of 1,2,4-triazolethiols

Sampa Sarkar #  1   2 , Sagar Swami #  2   3 , Sarvesh Kumar Soni  1 , Jessica K Holien  1 , Arshad Khan  2   4 , Arvind M Korwar  5 , Anjali P Likhite  6 , Ramesh A Joshi  6 , Rohini R Joshi  6 , Dhiman Sarkar  7   8
Affiliations

Detection of a target protein (GroEl2) in Mycobacterium tuberculosis using a derivative of 1,2,4-triazolethiols

Sampa Sarkar et al. Mol Divers. 2022 Oct.

Abstract

Herein, we identified a potent lead compound RRA2, within a series of 54 derivatives of 1,2,4-triazolethiols (exhibit good potency as an anti-mycobacterial agents) against intracellular Mycobacterium tuberculosis (Mtb). Compound RRA2 showed significant mycobactericidal activity against active stage Mycobacterium bovis BCG and Mtb with minimum inhibitory concentration (MIC) values of 2.3 and 2.0 µg/mL, respectively. At MIC value, RRA2 compound yielded 0.82 log reduction of colony-forming unit (cfu) against non-replicating Mtb. Furthermore, RRA2 compound was selected for further target identification due to the presence of alkyne group, showing higher selectivity index (> 66.66 ± 0.22, in non-replicating stage). Using "click" chemistry, we synthesized the biotin linker-RRA2 conjugate, purified with HPLC method and confirmed the conjugation of biotin linker-RRA2 complex by HR-MS analysis. Furthermore, we successfully pulled down and identified a specific target protein GroEl2, from Mtb whole-cell extract. Furthermore, computational molecular modeling indicated RRA2 could interact with GroEl2, which explains the structure-activity relationship observed in this study. GroEL-2 identified a potent and specific target protein for RRA 2 compound in whole cell extract of Mtb H37Ra.

Keywords: 1,2,4-triazolethiols; GroEl2; Mycobactericidal activity; Mycobacterium bovis BCG; Mycobacterium tuberculosis.

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