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Review
. 2021 Aug 9;12(11):1826-1838.
doi: 10.1039/d1md00170a. eCollection 2021 Nov 17.

Polarity-based fluorescence probes: properties and applications

Xiaojun Qin  1   2 , Xingye Yang  2 , Lupei Du  2 , Minyong Li  2   3
Affiliations
Review

Polarity-based fluorescence probes: properties and applications

Xiaojun Qin et al. RSC Med Chem. .

Abstract

Local polarity can affect the physical or chemical behaviors of surrounding molecules, especially in organisms. Cell polarity is the ultimate feedback of cellular status and regulation mechanisms. Hence, the abnormal alteration of polarity in organisms is closely linked with functional disorders and many diseases. It is incredibly significant to monitor and detect local polarity to explain the biological processes and diagnoses of some diseases. Because of their in vivo safe and real-time monitoring, several polarity-sensitive fluorophores and fluorescent probes have gradually emerged and been used in modern research. This review summarizes the fluorescence properties and applications of several representative polarity-sensitive fluorescent probes.

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Conflict of interest statement

There are no conflicts to declare.

Figures

Fig. 1
Fig. 1. Probes based on SBD and NBD (1–9) as well as coumarins (10–20).
Fig. 2
Fig. 2. Polarity-sensitive probes based on SBD and dansyl amine. (A) SBD-based probe 8 selectively detected the hERG channel and then showed fluorescence intensity depending on the hydrophobic interaction. (B) Dansyl amine-based probe 34 was used for the monitoring of hydrophobic interaction in Mcl-1 protein.
Fig. 3
Fig. 3. Probes based on ANS (21 and 22), dansyl amine (23–34), and coumarin (35–43) fluorophores.
Fig. 4
Fig. 4. Probes based on PRODAN (44–57) and neutral red (58–61).
Fig. 5
Fig. 5. Probes based on Nile red (62–70) and BODIPY (71–75).
None
Xiaojun Qin
None
Xingye Yang
None
Lupei Du
None
Minyong Li
See this image and copyright information in PMC

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