Effects of Bacillus amyloliquefaciens LFB112 on Growth Performance, Carcass Traits, Immune, and Serum Biochemical Response in Broiler Chickens

Abstract

This study aimed to investigate the effects of Bacillus amyloliquefaciens LFB112 on the growth performance, carcass traits, immune response, and serum biochemical parameters of broiler chickens. A total of 396 1 day old, mixed-sex commercial Ross 308 broilers with similar body weights were allotted into six treatment groups. The assigned groups were the CON group (basal diet with no supplement), AB (antibiotics) group (basal diet + 150 mg of aureomycin/kg), C+M group (basal diet + 5 × 10⁸ CFU/kg B. amyloliquefaciens LFB112 powder with vegetative cells + metabolites), C group (basal diet + 5 × 10⁸ CFU/kg B. amyloliquefaciens LFB112 vegetative cell powder with removed metabolites), M group (basal diet + 5 × 10⁸ CFU/kg B. amyloliquefaciens LFB112 metabolite powder with removed vegetative cells), and CICC group (basal diet + 5 × 10⁸ CFU/kg Bacillus subtilis CICC 20179). Results indicated that chickens in the C+M, C, and M groups had higher body weight (BW) and average daily gain (ADG) (p < 0.05) and lower feed conversion ratio (FCR) (p = 0.02) compared to the CON group. The C+M group showed the lowest abdominal fat rate compared to those in the CON, AB, and CICC groups (p < 0.05). Compared to the CON group, serum IgA and IgG levels in the C+M, C, and M groups significantly increased while declining in the AB group (p < 0.05). B. amyloliquefaciens LFB112 supplementation significantly reduced the serum triglyceride, cholesterol, urea, and creatinine levels, while increasing the serum glucose and total protein (p < 0.05). In conclusion, B. amyloliquefaciens LFB112 significantly improved the growth performance, carcass traits, immunity, and blood chemical indices of broiler chickens and may be used as an efficient broiler feed supplement.

Keywords: Bacillus amyloliquefaciens; broiler chickens; carcass traits; growth performance; immunity; serum chemical parameters.

Figure 1

Effect of Bacillus amyloliquefaciens LFB112 on immune organ index (g/kg) of broilers. (a) Effect of Bacillus amyloliquefaciens LFB112 on thymus. (b) Effect of Bacillus amyloliquefaciens LFB112 on bursa of Fabricius. (c) Effect of Bacillus amyloliquefaciens LFB112 on spleen. Experimental groups assigned were as follows: CON = basal diet; AB (antibiotics) = basal diet + aureomycin 150 mg/kg; C + M = basal diet + LFB112 fermentation dry powder with Bacillus cells + metabolites (5 × 10⁸ CFU/g); C = basal diet + LFB112 Bacillus cells powder with removed metabolites (5 × 10⁸ CFU/g); M = basal diet + LFB112 metabolite powder with removed Bacillus cells (5 × 10⁸ CFU/g); CICC = basal diet + Bacillus subtilis 20,179 (5 × 10⁸ CFU/g). (a–c) Different letters on standard error bars indicate a significant difference (p < 0.05). Data are shown as means and standard errors (n = 6).

Figure 2

Effect of Bacillus amyloliquefaciens LFB112 on serum immune factors (g/L) of broiler. Ig = immunoglobulin. (a) Effect of Bacillus amyloliquefaciens LFB112 on IgA. (b) Effect of Bacillus amyloliquefaciens LFB112 on IgG. (c) Effect of Bacillus amyloliquefaciens LFB112 on IgM. Experimental groups assigned were as follows: CON = basal diet; AB (antibiotics) = basal diet + aureomycin 150 mg/kg; C + M = basal diet + LFB112 fermentation dry powder with Bacillus cells + metabolites (5 × 10⁸ CFU/g); C = basal diet + LFB112 Bacillus cell powder with removed metabolites (5 × 10⁸ CFU/g); M = basal diet + LFB112 metabolite powder with removed Bacillus cells (5 × 10⁸ CFU/g); CICC = basal diet + Bacillus subtilis 20,179 (5 × 10⁸ CFU/g). (a–c) Different letters on standard error bars indicate a significant difference (p < 0.05). Data are shown as means and standard errors (n = 6).