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Review
. 2021 Nov 20;11(11):3319.
doi: 10.3390/ani11113319.

An Update on Semen Physiology, Technologies, and Selection Techniques for the Advancement of In Vitro Equine Embryo Production: Section II

Morgan F Orsolini  1 , Stuart A Meyers  2 , Pouya Dini  1
Affiliations
Review

An Update on Semen Physiology, Technologies, and Selection Techniques for the Advancement of In Vitro Equine Embryo Production: Section II

Morgan F Orsolini et al. Animals (Basel). .

Abstract

As the use of assisted reproductive technologies (ART) and in vitro embryo production (IVP) expand in the equine industry, it has become necessary to further our understanding of available semen selection techniques. This segment of our two-section review will focus on the selection of spermatozoa based on quality and sex for equine intracytoplasmic sperm injection (ICSI), as well as current and future developments in sperm sorting technologies. Ultimately, novel methods of semen selection will be assessed based on their efficacy in other species and their relevance and future application towards ARTs in the horse.

Keywords: assisted reproductive techniques; fertility; sperm; stallion.

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Conflict of interest statement

The authors have no conflict of interest to declare.

Figures

Figure 1
Figure 1
Conventional and novel methods of sperm selection. Selection techniques used to select superior quality sperm include (a) Swim Up (SU), (b) Density Gradient Centrifugation (DGC), (c) Density Gradient-Swim Up (DG-SU), (d) Glass Wool Filtration (GWF), (e) Microfluidics sorting (MF), (f) Fluorescence Activated Cell Sorting (FACS), (g) Magnetic Activated Cell Sorting (MACS), and (h) Zeta Potential Selection.
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