Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Nov 18;10(22):5389.
doi: 10.3390/jcm10225389.

Results of Proficiency Testing for Trichinella in Poland, 2015-2019

Mirosław Różycki  1 , Weronika Korpysa-Dzirba  1 , Aneta Bełcik  1 , Ewa Bilska-Zając  1 , Jacek Karamon  1 , Jacek Sroka  1 , Jolanta Zdybel  1 , Tomasz Cencek  1
Affiliations

Results of Proficiency Testing for Trichinella in Poland, 2015-2019

Mirosław Różycki et al. J Clin Med. .

Abstract

Trichinellosis is a zoonotic meat-borne disease caused by the nematodes of the genus Trichinella. Meat containing live Trichinella larvae is a source of infection. The examination of meat for Trichinella was introduced in 1869, but the digestion method for this did not appear in Poland until the late 1970s. Nowadays, the meat of all food animals susceptible to Trichinella spp. is examined in the frame of official post mortem control with the digestion method. The majority of laboratories in Poland meet the requirements of the ISO/IEC 17025 Standard (352 field laboratories). Laboratory personnel participate in quality control programs. This paper presents the results of proficiency tests (PTs) organized within 2015-2019 in Poland. Over this period, the laboratories examined 7580 samples (contamination levels: zero, one, three, and five larvae). Each laboratory was provided with a set of samples (one negative and three positive). Over 95% of the samples were considered correct in qualitative assessments, though the results of the quantitative evaluations were slightly lower, with 89% of samples being considered correct. Based on a sample evaluation, 88% of laboratories passed the PT comparison. A slight decrease was observed in the examination of samples spiked with five larvae, and great progress was achieved in samples containing three larvae. Low levels of sample contamination are sought after in laboratories but may make evaluations difficult. For this reason, we must consider increasing the number of larvae added to the samples in the next PTs.

Keywords: Poland; Trichinella spiralis; proficiency testing.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
The percentage of correct results in particular years and the formation of a trend.
Figure 2
Figure 2
The percentage of samples assessed as correct, doubtful, and incorrect in quantitative assessment.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Sobolewski J. History of the production and application of veterinary medications and biological preparations in Poland from the Nineteenth Century to 1945. Vet. Med. 2019;75:318–320. doi: 10.21521/mw.6231. - DOI
    1. Liciardi M., Marucci G., Addis G., Ludovisi A., Morales M.A.G., Deiana B., Cabaj W., Pozio E. Trichinella britovi and Trichinella spiralis mixed infection in a horse from Poland. Vet. Parasitol. 2009;161:345–348. doi: 10.1016/j.vetpar.2009.01.013. - DOI - PubMed
    1. Flis M., Grela E.R., Gugala D. Epizootic and epidemiological situation of Trichinella sp infection in Poland in 2006–2015 in view of wild boar population dynamics. J. Vet. Res. 2017;61:181–187. doi: 10.1515/jvetres-2017-0023. - DOI - PMC - PubMed
    1. Bilska-Zajac E., Rozycki M., Antolak E., Belcik A., Gradziel-Krukowska K., Karamon J., Sroka J., Zdybel J., Cencek T. Occurrence of Trichinella spp. in rats on pig farms. Ann. Agric. Environ. Med. 2018;25:698–700. doi: 10.26444/aaem/99555. - DOI - PubMed
    1. Marucci G., Tonanzi D., Cherchi S., Galati F., Bella A., Interisano M., Ludovisi A., Amati A., Pozio E. Proficiency testing to detect Trichinella larvae in meat in the European Union. Vet. Parasitol. 2016;231:145–149. doi: 10.1016/j.vetpar.2016.04.009. - DOI - PubMed

LinkOut - more resources