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. 2022:2393:417-436.
doi: 10.1007/978-1-0716-1803-5_21.

Optical and Electrochemical Aptasensors for Sensitive Detection of Aflatoxin B1 and Aflatoxin M1 in Blood Serum, Grape Juice, and Milk Samples

Mohammad Ramezani  1   2 , Seyed Hamid Jalalian  3   4   5 , Seyed Mohammad Taghdisi  6 , Khalil Abnous  3   7 , Mona Alibolandi  3   8
Affiliations

Optical and Electrochemical Aptasensors for Sensitive Detection of Aflatoxin B1 and Aflatoxin M1 in Blood Serum, Grape Juice, and Milk Samples

Mohammad Ramezani et al. Methods Mol Biol. 2022.

Abstract

Aflatoxin food contamination with toxic and carcinogenic impacts on human health is a global concern. We have developed aptasensors for the detection of Aflatoxin M1 (AFM1) and B1 (AFB1) using electrochemical and optical methods. In the first method, an electrochemical aptasensor was designed for the detection of AFM1 based on complementary strand of AFM1 aptamer that was attached onto the gold nanoparticles and a hairpin-shaped AFM1 aptamer. The designed electrochemical aptasensor showed high selectivity toward AFM1 with a limit of detection (LOD) as low as 0.9 nM. Moreover, the developed aptasensor was successfully used to detect AFM1 in milk and serum with LODs of 1.8 and 1.2 nM, respectively. In the second method, a novel electrochemical aptasensor was developed based on the π-shape structure of AFB1 aptamer. The detection limit was found to be 2 pg/mL in buffer. Also, the developed aptasensor was used to analyze AFB1 spiked human serum and grape juice samples, and the recoveries were 95.4-108.1%. In another method, a fluorescent sensing scheme was developed for AFB1 detection based on a hairpin structure of G-quadruplex oligonucleotide-aptamer chimera, streptavidin-coated silica nanoparticles (SNP-streptavidin) and N-methyl mesoporphyrin IX (NMM). The LOD was reported as 8 pg/mL with a linear range of 30-900 pg/mL. Moreover, the developed sensor could detect AFB1 in serum and grape juice with the LOD of 9.8 and 11.2 pg/mL, respectively.

Keywords: Aflatoxin B1; Aflatoxin M1; Aptamer; Aptasensor; Exonuclease I; Fluorescence; π-shape structure.

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