Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2021 Nov 4;18(3):e20210031.
doi: 10.1590/1984-3143-AR2021-0031. eCollection 2021.

Meta-analysis of melatonin treatment and porcine somatic cell nuclear transfer embryo development

Zhenhua Guo  1 , Wengui Chen  2 , Lei Lv  3 , Di Liu  1
Affiliations
Review

Meta-analysis of melatonin treatment and porcine somatic cell nuclear transfer embryo development

Zhenhua Guo et al. Anim Reprod. .

Abstract

Porcine somatic cell nuclear transfer (SCNT) plays an important role in many areas of research. However, the low efficiency of SCNT in porcine embryos limits its applications. Porcine embryos contain high concentrations of lipid, which makes them vulnerable to oxidative stress. Some studies have used melatonin to reduce reactive oxygen species damage. At present there are many reports concerning the effect of exogenous melatonin on porcine SCNT. Some studies suggest that the addition of melatonin can increase the number of blastocyst cells, while others indicate that melatonin can reduce the number of blastocyst cells. Therefore, a meta-analysis was carried out to resolve the contradiction. In this study, a total of 63 articles from the past 30 years were analyzed, and six papers were finally selected. Through the analysis, it was found that the blastocyst rate was increased by adding exogenous melatonin. Melatonin had no effect on cleavage rate or the number of blastocyst cells, but did decrease the number of apoptotic cells. This result is crucial for future research on embryo implantation.

Keywords: SCNT; melatonin; meta-analysis; porcine.

PubMed Disclaimer

Conflict of interest statement

Conflict of interest: The authors have no conflict of interest to declare.

Figures

Figure 1
Figure 1. Summary of study selection.
Figure 2
Figure 2. Forest plot of melatonin treatment effects on (A) porcine blastocyst rate and (B) cleavage rate, CI = 95%. (C) funnel plot of melatonin treatment effects on porcine blastocyst rate. “Liang 10 ^ - 6” indicates that the additive amount is 10 ^ - 6 in Liang's study; “Nakano 144h-a” refers to group A with 144-hour culture time in Nakano's study; “Pang donor 10 ^ - 6” refers to the treatment of the donor in Pang's study with an additive amount of 10 ^ - 6; and “all” refers to the treatment of donor and embryo in Pang's study.
Figure 3
Figure 3. Forest plot of melatonin treatment effects on (A) porcine blastocyst cell number and (B) blastocyst apoptotic cell number. CI = 95%.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Abecia JA, Forcada F, Zúñiga O. The effect of melatonin on the secretion of progesterone in sheep and on the development of ovine embryos. Vet Res Commun. 2002;26(2):151–158. doi: 10.1023/A:1014099719034. - DOI - PubMed
    1. An Q, Peng W, Cheng Y, Lu Z, Zhou C, Zhang Y, Su J. Melatonin supplementation during in vitro maturation of oocyte enhances subsequent development of bovine cloned embryos. J Cell Physiol. 2019;234(10):17370–17381. doi: 10.1002/jcp.28357. - DOI - PubMed
    1. Berlinguer F, Leoni GG, Succu S, Spezzigu A, Madeddu M, Satta V, Bebbere D, Contreras-Solis I, Gonzalez-Bulnes A, Naitana S. Exogenous melatonin positively influences follicular dynamics, oocyte developmental competence and blastocyst output in a goat model. J Pineal Res. 2009;46(4):383–391. doi: 10.1111/j.1600-079X.2009.00674.x. - DOI - PubMed
    1. Chen Y-C, Sheen J-M, Tiao M-M, Tain Y-L, Huang L-T. Roles of melatonin in fetal programming in compromised pregnancies. Int J Mol Sci. 2013;14(3):5380–5401. doi: 10.3390/ijms14035380. - DOI - PMC - PubMed
    1. Choi J, Park S-M, Lee E, Kim J-H, Jeong YI, Lee J-Y, Park S-W, Kim HS, Hossein M, Jeong Y-W, Kim S, Hyun SH, Hwang WS. Anti-apoptotic effect of melatonin on preimplantation development of porcine parthenogenetic embryos. Mol Reprod Dev. 2008;75(7):1127–1135. doi: 10.1002/mrd.20861. - DOI - PubMed

LinkOut - more resources