New insights about the EptA protein and its correlation with the pmrC gene in polymyxin resistance in Pseudomonas aeruginosa

Abstract

Nowadays, clinical and scientific interest in antibiotics, as polymyxin, has increased due to the large number of reports of multiresistant Gram-negative bacteria, as Pseudomonas aeruginosa. The aim of this study was to investigate a related group of proteins for resistance to polymyxins, encoded by P. aeruginosa genome, through in silico analysis. The mobilized colistin resistance 1 (MCR₁) protein from Escherichia coli was used for comparison. Similar sequences to the protein MCR₁ in P. aeruginosa were analysed for physicochemical properties. 31 protein isoforms in P. aeruginosa (EptA) were found able to confer resistance to polymyxin showing protein lengths between 551 and 572 amino acids, with molecular mass values between 61.36 - 62. 80 kDa, isoelectric point between 6.10 to 7.17, instability index between 33.76 to 41.87, aliphatic index between 98.67 to 102.63 and the hydropathyindex between - 0.008 to 0.094. These proteins belong to the DUF₁₇₀₅ superfamily with bit-score values between 559.81 and 629.78. A high degree of similarity between EpTAs in P. aeruginosa was observed in relation to other proteins that confer resistance to polymyxins, present in Gram-negative bacteria species of clinical interest. Although, further studies are needed to identify the actual contribution of EptAs in P. aeruginosa species.

Keywords: Bacterial resistance; Computational biology; Gram-negative bacteria; Polymyxin.

Fig. 1

Cladogram generated by the Neighbor-Joining method employing alignment and multiple performance with Molecular Evolutionary Genetics Analysis software - MEGA X. Proteins belonging to the DUF₁₇₀₅ superfamily of P. aeruginosa and proteins responsible for polymyxin resistance in Gram-negative bacteria with clinical interest. Node values = bootstrap test (1000 pseudo-replicas).