Biocompatibility, antioxidant activity and collagen photoprotection properties of C60 fullerene adduct with L-methionine

Abstract

Functionalization of the fullerene core with amino acids has become a new and promising direction in the field of nanochemistry. The biologic activity of water-soluble fullerene derivatives is based on such properties as lipophilicity, electron deficiency and photosensitivity. The complex of above-mentioned properties can be used to develop protection of biomolecules (in particular, proteins) from external physical and chemical influences. Thus, development and up-scaling of synthesis procedures, as well as investigation of the biological properties of these derivatives, are extremely important. This paper presents new data on the biocompatibility studies of C₆₀ fullerene adduct with L-methionine (C₆₀[C₅H₁₁NO₂S]₃; C₆₀-Met). Antiradical activity, binding to human serum albumin (HSA), collagen and deoxyribonucleic acid (DNA), hemocompatibility, photodynamic properties, genotoxicity and cytotoxicity were studied. In addition, it was found that C₆₀-Met increases the photostability of the collagen molecule, and this effect is dose-dependent.

Keywords: Biological activity; Collagen; Fullerenes; L-Methionine; Ultra-violet light.