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. 2021 Nov;25(Suppl 2):S114-119.
doi: 10.5213/inj.2142350.175. Epub 2021 Nov 30.

Liberation of Serotonin Is Not Unaffected by Acetylcholine in Rat Hippocampus

Jae Heon Kim  1 , Young Soo Ahn  2 , Yun Seob Song  1
Affiliations

Liberation of Serotonin Is Not Unaffected by Acetylcholine in Rat Hippocampus

Jae Heon Kim et al. Int Neurourol J. 2021 Nov.

Abstract

Purpose: Raised cerebral titers of acetylcholine have notable links with storage symptomatology related to lower urinary tract symptoms. The hippocampus contributes to the normal control of continence in the majority of instances (circuit 3). Owing to synaptic connections with other nerve cells, acetylcholine affects the micturition pathway via the liberation of additional cerebral neurotransmitters. Despite the fact that cerebral serotonin is a key inhibitor of reflex bladder muscle contractions, the influence of acetylcholine on its liberation is poorly delineated. The current research was conducted in order to explore the role of acetylcholine in serotonin liberation from sections of rat hippocampus in order to improve the comprehension of the relationship between cholinergic and serotonergic neurons.

Methods: Hippocampal sections from 6 mature male Sprague-Dawley rats were equilibrated over a 30-minute period in standard incubation medium so as to facilitate [3H]5-hydroxytryptamine (5-HT) uptake. The cerebral neurotransmitter, acetylcholine, was applied to the sections. Aliquots of drained medium solution were utilized in order to quantify the radioactivity associated with [3H]5-HT liberation; any alterations in this parameter were noted.

Results: When judged against the controls, [3H]5-HT liberation from the hippocampal sections remained unaltered following the administration of acetylcholine, implying that this agent has no inhibitory action on this process.

Conclusion: Serotonin liberation from murine hippocampal sections is unaffected by acetylcholine. It is postulated that the bladder micturition reflex responds to acetylcholine through its immediate cholinergic activity rather than by its influence on serotonin release. These pathways are a promising target for the design of de novo therapeutic agents.

Keywords: Acetylcholine; Hippocampal slices; Serotonin.

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Conflict of interest statement

Conflict of Interest

No potential conflict of interest relevant to this article was reported.

Figures

Fig. 1.
Fig. 1.
Spontaneous release of [3H]5-hydroxytryptamine.
Fig. 2.
Fig. 2.
Effect of acetylcholine(10-5M) on the % change of 5-hydroxytryptamine (5-HT) release.
See this image and copyright information in PMC

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