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. 2022:2316:143-151.
doi: 10.1007/978-1-0716-1464-8_13.

Detection of Viroids by RT-PCR

Nerida J Donovan  1 , Grant A Chambers  2 , Mengji Cao  3
Affiliations

Detection of Viroids by RT-PCR

Nerida J Donovan et al. Methods Mol Biol. 2022.

Abstract

Reverse transcription-polymerase chain reaction (RT-PCR) is an effective method for detecting the presence of viroids in plant tissue. Viroid RNA is converted to cDNA and amplified to detectable levels, making it a fast and useful detection tool, even when the viroid is present at low levels. Methods of viroid detection using conventional RT-PCR are described in this chapter.

Keywords: Gel electrophoresis; Primers; Reverse transcription-polymerase chain reaction (RT-PCR); Viroid detection.

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