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. 2021 Oct;37(5):489-493.
doi: 10.5423/PPJ.NT.05.2021.0078. Epub 2021 Oct 1.

Identification of strA-strB Genes in Streptomycin-Resistant Pseudomonas syringae pv. actinidiae Biovar 2 Strains Isolated in Korea

Young Sun Lee  1 , Gyoung Hee Kim  2 , Young Jin Koh  2 , Jae Sung Jung  1
Affiliations

Identification of strA-strB Genes in Streptomycin-Resistant Pseudomonas syringae pv. actinidiae Biovar 2 Strains Isolated in Korea

Young Sun Lee et al. Plant Pathol J. 2021 Oct.

Abstract

Bacterial canker is a devastating disease of kiwifruit caused by the bacterium Pseudomonas syringe pv. actinidiae. Canker disease of kiwifruit in Korea has been controlled using streptomycin for more than two decades. Four streptomycin-resistant strains, belonging to biovar 2, which are found only in Korea, were collected between 2013 and 2014 from different orchards located in Jeju, Korea. The genetic background for streptomycin resistance among P. syringe pv. actinidiae strains were determined by examining the presence of strA-strB or aadA, which are genes frequently found in streptomycin-resistant bacteria, and a point mutation at codon 43 in the rpsL gene. All four streptomycin-resistant strains of P. syringe pv. actinidiae investigated in this study contained strA-strB as a resistant determinant. The presence of the aadA gene and a mutation in codon 43 of the rpsL gene was not identified.

Keywords: Pseudomonas syringe pv. actinidiae; bacterial canker; kiwifruit; streptomycin resistance.

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Conflict of interest statement

Conflicts of Interest

No potential conflict of interest relevant to this article was reported.

Figures

Fig. 1
Fig. 1
PCR detection of strA (A), strB (B), strA-strB (C), and aadA (D) genes. Lane M, size markers (100 bp ladder); lanes 1–5, strains of Pseudomonas syringae pv. actinidia: KSH1351 (lane 1), OYJ1351 (lane 2), KTS1431 (lane 3), KBD14311 (lane 4), and JJ18 (lane 5); lane 6–7, strains of Escherichia coli: DNRP545 (lane 6) and DMRP5410 (lane 7).
See this image and copyright information in PMC

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