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. 2021 Oct 19;45(5):1454-1462.
doi: 10.3906/kim-2104-13. eCollection 2021.

Antiinfective properties of ursolic acid-loaded chitosan nanoparticles against Staphylococcus aureus

Fatemeh Ghasemzadeh  1 , Ghasem D Najafpour  1 , Maedeh Mohammadi  1
Affiliations

Antiinfective properties of ursolic acid-loaded chitosan nanoparticles against Staphylococcus aureus

Fatemeh Ghasemzadeh et al. Turk J Chem. .

Abstract

The present study aimed to synthesize ursolic acid-loaded chitosan nanoparticles (UA-Ch-NPs) as an antiinfective agent against 21 Staphylococcus aureus isolates. The UA-Ch-NPs were synthesized by a simple method and then characterized by TEM, FTIR, DLS-zeta potential, and XRD analyses. According to the characterization results, highly dispersed spherical nanoparticles with a mean diameter of 258 nm and a zeta potential of + 40.1 mV were developed. The antibacterial properties of UA-Ch-NPs were investigated and their inhibitory effect on biofilm formation was demonstrated by AFM. Finally, the expression levels of icaA and icaD were measured using real-time PCR. Results indicated that the minimum inhibitory concentration (MIC) of UA and UA-Ch-NPs against S. aureus was 64 and 32 µg/mL, respectively. The treatment of bacterial cells with UA-Ch-NPs significantly decreased the expression of icaA and icaD genes which are engaged in biofilm formation. Our results indicated that UA-Ch-NPs could be a promising material for antibacterial and antibiofilm applications.

Keywords: biofilm; Staphylococcus aureus bacteria; Ursolic acid-loaded chitosan nanoparticle.

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Conflict of interest statement

CONFLICT OF INTEREST: none declared

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