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Comparative Study
. 2021 Dec;172(2):245-249.
doi: 10.1007/s10517-021-05370-6. Epub 2021 Dec 2.

Comparative Study of the γH2AX Foci Forming in Human Lung Fibroblasts Incubated in Media Containing Tritium-Labeled Thymidine or Amino Acids

S M Rodneva  1 , A A Osipov  1   2 , D V Guryev  3   4 , A A Tsishnatti  1 , Y А Fedotov  1   2 , E I Yashkina  1 , N Y Vorobyova  1   2 , A A Maksimov  1 , O A Kochetkov  1 , A S Samoylov  1 , A N Osipov  1   2
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Comparative Study

Comparative Study of the γH2AX Foci Forming in Human Lung Fibroblasts Incubated in Media Containing Tritium-Labeled Thymidine or Amino Acids

S M Rodneva et al. Bull Exp Biol Med. 2021 Dec.

Abstract

We compared the formation of γH2AX foci (marker of DNA double-strand breaks) in human lung fibroblasts (MRC-5 line) during their 24-h incubation in a medium containing 3H-labeled thymidine or amino acids (glycine, alanine, and proline) with specific radioactivity from 100 to 400 MBq/liter. A linear dependence of changes in the number of γH2AX foci on the specific radioactivity of the medium was revealed. The quantitative yield of DNA double-strand breaks under the influence of 3H-thymidine was more than 2-fold higher than under the influence of 3H-labeled amino acids. Comparative analysis of the yields of DNA double-strand breaks during cell incubation with 3H-labeled amino acids showed that 3H-alanine produced more pronounced effect that 3H-proline, which is consistent with the data on the content of their non-radioactive analogs in chromatin proteins.

Keywords: 3H-amino acids; 3H-thymidine; MRC-5; tritium; γH2AX.

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