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. 2022:2402:21-30.
doi: 10.1007/978-1-0716-1843-1_3.

Langmuir-Schaefer Deposition to Create an Asymmetrical Lipopolysaccharide Sparsely Tethered Lipid Bilayer

Charles G Cranfield  1 , Anton P Le Brun  2 , Alvaro Garcia  3 , Bruce A Cornell  3   4 , Stephen A Holt  3   2
Affiliations

Langmuir-Schaefer Deposition to Create an Asymmetrical Lipopolysaccharide Sparsely Tethered Lipid Bilayer

Charles G Cranfield et al. Methods Mol Biol. 2022.

Abstract

Because they are firmly anchored to a noble metal substrate, tethered bilayer lipid membranes (tBLMs) are considerably more robust than supported lipid bilayers such as black lipid membranes (BLMs) (Cranfield et al. Biophys J 106:182-189, 2014). The challenge to rapidly create asymmetrical tBLMs that include a lipopolysaccharide outer leaflet for bacterial model membrane research can be overcome by the use of a Langmuir-Schaefer deposition protocol. Here, we describe the procedures required to assemble and test asymmetric lipopolysaccharide (LPS) tethered lipid bilayers.

Keywords: AC Impedance Spectroscopy; Asymmetric Bilayers; Langmuir-Schaefer deposition; Tethered Bilayer Lipid Membranes.

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References

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    1. Cranfield C, Carne S, Martinac B, Cornell B (2015) The assembly and use of tethered bilayer lipid membranes (tBLMs). In: Methods in membrane lipids. Springer, New York, pp 45–53
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