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. 2021 Aug;154(2):391-398.
doi: 10.4103/ijmr.IJMR_1111_19.

Polymorphism of interleukin-6 -174 G/C (rs1800795) & the corresponding interleukin-6 level as a prognostic marker of cervical cancer

Priyanka Wagh  1 , Priyanka Kulkarni  1 , Shilpa Kerkar  1 , Hemant Tongaonkar  2 , Hemangi Chaudhari  3 , Himangi Warke  3 , Kedar Deodhar  2 , Bharat Rekhi  2 , Jayanti Mania-Pramanik  1
Affiliations

Polymorphism of interleukin-6 -174 G/C (rs1800795) & the corresponding interleukin-6 level as a prognostic marker of cervical cancer

Priyanka Wagh et al. Indian J Med Res. 2021 Aug.

Abstract

Background & objectives: Aetiology of cervical cancer (CaCx) is multifactorial. Besides human papillomavirus (HPV) infection, many immunogenetic factors are involved in this complex process. The present study was carried out to investigate one such factor, interleukin-6 (IL-6), a central pro-inflammatory cytokine and a polymorphism at its promoter region -174 G/C (rs1800795) with CaCx.

Methods: HPV-infected women with or without CaCx were enrolled in group I and II, respectively. Another group of uninfected healthy women was also included as group III for comparison. Polymorphism in IL-6-174 G/C and IL-6 levels were analyzed by sequence-specific primer PCR (PCR-SSP) and ELISA, respectively.

Results: Groups I (n=111) and II (n=87) had significantly higher frequency of IL-6-174 GG genotype [odds ratios (OR)=3.9; P<0.001 and OR=3.2; P<0.001, respectively] as compared to group III (n=163). Furthermore, individuals with GG or GC genotypes had high IL-6 levels than those with CC genotypes. IL-6 levels were significantly (P<0.001) elevated in group I. This was also significantly high in untreated cases as compared to treated (P<0.05) ones. IL-6 levels of treated group were comparable with groups II and III.

Interpretation & conclusions: Our results suggested a possible association of IL-6-174 GG with CaCx, which was also associated with high IL-6 levels. Decreased levels of IL-6 following treatment indicate its possible prognostic use in CaCx cases.

Keywords: Cervical cancer; human papillomavirus; interleukin-6; prognostic marker; serum interleukin-6; single-nucleotide polymorphism.

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Conflict of interest statement

None

Figures

Fig. 1
Fig. 1
Interleukin-6-174 G/C single-nucleotide polymorphism (SNP) using commercial cytokine genotyping kit: Genotyping was done for an array of 22 SNPs using the commercially available kit. Two interleukin-6 single-nucleotide polymorphisms were part of the panel.
Fig. 2
Fig. 2
IL-6 serum levels with mean±SEM (pg/ml) in cervical cancer groups (untreated vs treated), HPV positive and control groups. (Untreated vs treated, P=0.032; untreated vs HPV positives and controls, P<0.001; treated vs control, P=0.25).
See this image and copyright information in PMC

References

    1. World Health Organization. Global cancer observatory (GCO) data for cervical cancer. [assessed on October 10, 2020]. Available from:https://gco.iarc.fr/today/online-analysis-multibars?v=2020&mode=cancer&m... .
    1. Stanley M. Pathology and epidemiology of HPV infection in females. Gynecol Oncol. 2010;117:S5–10. - PubMed
    1. Zur Hausen H. Papillomaviruses and cancer:From basic studies to clinical application. Nat Rev Cancer. 2002;2:342–50. - PubMed
    1. Wang SS, Hildesheim A. Chapter 5:Viral and host factors in human papillomavirus persistence and progression. J Natl Cancer Inst Monogr. 2003;31:35–40. - PubMed
    1. Fishman D, Faulds G, Jeffery R, Mohamed-Ali V, Yudkin JS, Humphries S, et al. The effect of novel polymorphisms in the interleukin-6 (IL-6) gene on IL-6 transcription and plasma IL-6 levels, and an association with systemic-onset juvenile chronic arthritis. J Clin Invest. 1998;102:1369–76. - PMC - PubMed