Partial purification and characterization of a recombinase from human cells
- PMID: 3485474
- DOI: 10.1016/0092-8674(86)90011-5
Partial purification and characterization of a recombinase from human cells
Abstract
We describe the partial purification and characterization of a human recombinase activity from RPMI 1788 B lymphoblasts. Stoichiometric amounts of recombinase carry out a strand transfer reaction between linear duplex DNA and homologous circular single-strand DNA. The product of strand transfer by the recombinase is a joint molecule composed of a single-strand circle joined to one end of the linear duplex molecule by a region of DNA heteroduplex at least 150 bp long. Formation of DNA heteroduplexes is accompanied by strand displacement. Strand invasion initiates at the ends of the linear duplex. Finally, strand displacement by human recombinase exhibits polarity and proceeds in a 3' to 5' direction. This is the first demonstration of a strand transfer activity from a high eukaryote. We discuss similarities between our recombinase and the RecA and rec1 recombination proteins from E. coli and Ustilago maydis, respectively.
Similar articles
-
Partial purification of an activity from human cells that promotes homologous pairing and the formation of heteroduplex DNA in the presence of ATP.Mol Gen Genet. 1987 Jun;208(1-2):10-4. doi: 10.1007/BF00330415. Mol Gen Genet. 1987. PMID: 2956479
-
Heteroduplex formation and polarity during strand transfer promoted by Ustilago rec 1 protein.Cell. 1983 Jul;33(3):857-64. doi: 10.1016/0092-8674(83)90028-4. Cell. 1983. PMID: 6871997
-
Formation of joint DNA molecules by two eukaryotic strand exchange proteins does not require melting of a DNA duplex.J Biol Chem. 1989 Mar 25;264(9):5089-97. J Biol Chem. 1989. PMID: 2538473
-
Homologous genetic recombination as an intrinsic dynamic property of a DNA structure induced by RecA/Rad51-family proteins: a possible advantage of DNA over RNA as genomic material.Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8425-32. doi: 10.1073/pnas.111005198. Proc Natl Acad Sci U S A. 2001. PMID: 11459985 Free PMC article. Review.
-
Assembly of RecA-like recombinases: distinct roles for mediator proteins in mitosis and meiosis.Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8411-8. doi: 10.1073/pnas.121046198. Proc Natl Acad Sci U S A. 2001. PMID: 11459983 Free PMC article. Review.
Cited by
-
Phenol-treatment and a homologous pairing-assay.Nucleic Acids Res. 1992 Jul 25;20(14):3679-84. doi: 10.1093/nar/20.14.3679. Nucleic Acids Res. 1992. PMID: 1641333 Free PMC article.
-
Partial purification of an activity from human cells that promotes homologous pairing and the formation of heteroduplex DNA in the presence of ATP.Mol Gen Genet. 1987 Jun;208(1-2):10-4. doi: 10.1007/BF00330415. Mol Gen Genet. 1987. PMID: 2956479
-
Nucleosomes on linear duplex DNA allow homologous pairing but prevent strand exchange promoted by RecA protein.Proc Natl Acad Sci U S A. 1991 Feb 15;88(4):1344-8. doi: 10.1073/pnas.88.4.1344. Proc Natl Acad Sci U S A. 1991. PMID: 1996335 Free PMC article.
-
Characterization of nonconservative homologous junctions in mammalian cells.Mol Cell Biol. 1990 Dec;10(12):6613-8. doi: 10.1128/mcb.10.12.6613-6618.1990. Mol Cell Biol. 1990. PMID: 2174111 Free PMC article.
-
The search for the right partner: homologous pairing and DNA strand exchange proteins in eukaryotes.Experientia. 1994 Mar 15;50(3):223-33. doi: 10.1007/BF01924005. Experientia. 1994. PMID: 8143796 Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
