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. 2021 Dec;172(2):169-174.
doi: 10.1007/s10517-021-05357-3. Epub 2021 Dec 2.

Effect of Pregnancy Specific β1-Glycoprotein on the Replicative Potential of Naïve T Cells and Immune Memory T Cells

V P Timganova  1 , L S Litvinova  2 , K A Yurova  2 , O G Khaziakhmatova  2 , M S Bochkova  1 , P V Khramtsov  1   2 , M B Raev  1 , S A Zamorina  3
Affiliations

Effect of Pregnancy Specific β1-Glycoprotein on the Replicative Potential of Naïve T Cells and Immune Memory T Cells

V P Timganova et al. Bull Exp Biol Med. 2021 Dec.

Abstract

We studied the effects of pregnancy-specific β1-glycoprotein (PSG) on the replicative potential of naïve T cells (CD45RA+) and immune memory T cells (CD45R0+) in vitro by evaluating the expression of the hTERT gene in combination with the proliferative activity of cells. Human PSG was obtained by the author's patented method of immunopurification using a biospecific sorbent with subsequent removal of immunoglobulin contamination on a HiTrap Protein G HP column. We used monocultures of CD45RA+ and CD45R0+ lymphocytes isolated from peripheral blood mononuclear cells of reproductive-age women. It was found that PSG in physiological concentrations inhibited the expression of the hTERT gene mRNA in naïve T cells and immune memory T cells and simultaneously reduced the number of proliferating T cells estimated by the differential gating method. At the same time, PSG reduced CD71 expression only on naïve T cells without affecting this molecule on immune memory T cells. Thus, PSG decreased the replication potential and suppressed the proliferation of T cells and immune memory T cells, which in the context of pregnancy can contribute to the formation of immune tolerance to the semi-allogeneic embryo.

Keywords: hTERT; memory T cells; naïve T cells; pregnancy-specific β1-glycoprotein; proliferation.

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