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. 2021 Nov 24:14:8753-8762.
doi: 10.2147/IJGM.S337909. eCollection 2021.

Blood Levels of Glutamine and Nitrotyrosine in Patients with Chronic Viral Hepatitis

Hussam Murad  1   2 , Haythum Tayeb  3 , Mahmoud Mosli  4 , Misbahuddin Rafeeq  1 , Mohammed Basheikh  5
Affiliations

Blood Levels of Glutamine and Nitrotyrosine in Patients with Chronic Viral Hepatitis

Hussam Murad et al. Int J Gen Med. .

Abstract

Purpose: Oxidative stress is involved in pathogenesis of chronic viral hepatitis. Glutamine is an antioxidant, but there is a controversy about its risk-benefits. Nitrotyrosine is an oxidative stress marker. This observational cross-sectional study was designed to compare blood levels of glutamine and nitrotyrosine in treated versus untreated chronic viral hepatitis patients.

Patients and methods: Five groups (n = 250) were included: hepatitis B untreated (HBV), hepatitis C untreated (HCV), HBV treated (HBVT), and HCV treated (HCVT) groups plus a normal control group. Liver function tests and blood levels of glutamine, nitrotyrosine, viral loads, and HBsAg were measured.

Results: Blood levels of glutamine and nitrotyrosine in all patient groups significantly increased compared with normal controls with non-significant differences in-between. Both tests showed significant large correlations with HBV-DNA or HCV-RNA test positivity, high accuracies, and cutoff scores with high sensitivities and specificities. The viral loads and HBsAg levels were significantly lower in treated versus untreated groups. However, they poorly correlated with levels of glutamine and nitrotyrosine in all patient groups.

Conclusion: Blood levels of glutamine and nitrotyrosine significantly increased in treated and untreated chronic viral hepatitis B and C patients compared with normal controls. Both tests showed high accuracies and cutoff scores with high sensitivities and specificities. However, they did not differ significantly in treated versus untreated patients. To our knowledge, this is the first data showing elevation of glutamine and nitrotyrosine in treated and untreated chronic viral hepatitis. A prospective longitudinal study with repeated measurements of glutamine and nitrotyrosine is recommended to verify if they can predict response to treatment. Study of other oxidative stress markers is also advised to clarify if the elevated nitrotyrosine could be an oxidative stress marker in these patients, and whether the increased glutamine could act as an antioxidant or as a predictive agent for deleterious consequences.

Keywords: glutaminolysis; nitrotyrosination; oxidant stress.

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Conflict of interest statement

The authors declare that they have no conflicts of interest in this work.

Figures

Figure 1
Figure 1
The receiver operating characteristic (ROC) curve for glutamine (GLN) and nitrotyrosine (NT) detection of HBV infection according to positive DNA testing in the patient groups: (A) GLU-HBV, (B) NT-HBV, (C) GLU-HBVT, and (D) NT-HBVT.
Figure 2
Figure 2
The receiver operating characteristic (ROC) curve for glutamine (GLN) and nitrotyrosine (NT) detection of HCV infection according to positive RNA testing in the patient groups: (A) GLU-HCV, (B) NT-HCV, (C) GLU-HCVT, and (D) NT-HCVT.
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