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. 2022 Jan;36(1):e24157.
doi: 10.1002/jcla.24157. Epub 2021 Dec 3.

The possible mechanism of Hippophae fructus oil applied in tympanic membrane repair identified based on network pharmacology and molecular docking

Juntao Huang  1   2 , Bing Mei Teh  3   4   5 , Ziqian Xu  6 , Zhechen Yuan  1   2 , Chongchang Zhou  1   2 , Yunbin Shi  1   2 , Yi Shen  1   2
Affiliations

The possible mechanism of Hippophae fructus oil applied in tympanic membrane repair identified based on network pharmacology and molecular docking

Juntao Huang et al. J Clin Lab Anal. 2022 Jan.

Abstract

Objective: This study aimed to explore the mechanisms of Hippophae fructus oil (HFO) in the treatment of tympanic membrane (TM) perforation through network pharmacology-based identification.

Methods: The compounds and related targets of HFO were extracted from the TCMSP database, and disease information was obtained from the OMIM, GeneCards, PharmGkb, TTD, and DrugBank databases. A Venn diagram was generated to show the common targets of HFO and TM, and GO and KEGG analyses were performed to explore the potential biological processes and signaling pathways. The PPI network and core gene subnetwork were constructed using the STRING database and Cytoscape software. A molecular docking analysis was also conducted to simulate the combination of compounds and gene proteins.

Results: A total of 33 compounds and their related targets were obtained from the TCMSP database. After screening the 393 TM-related targets, 21 compounds and 22 gene proteins were selected to establish the network diagram. GO and KEGG enrichment analyses revealed that HFO may promote TM healing by influencing cellular oxidative stress and related signaling pathways. A critical subnetwork was obtained by analyzing the PPI network with nine core genes: CASP3, MMP2, IL1B, TP53, EGFR, CXCL8, ESR1, PTGS2, and IL6. In addition, a molecular docking analysis revealed that quercetin strongly binds the core proteins.

Conclusion: According to the analysis, HFO can be utilized to repair perforations by influencing cellular oxidative stress. Quercetin is one of the active compounds that potentially plays an important role in TM regeneration by influencing 17 gene proteins.

Keywords: Hippophae fructus; molecular docking; network pharmacology; tympanic membrane perforation; wound healing.

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Conflict of interest statement

None.

Figures

FIGURE 1
FIGURE 1
Venn diagram of gene intersections between HFO and TM perforation. HFO, Hippophae fructus oil; TM, tympanic membrane
FIGURE 2
FIGURE 2
HFO‐compounds‐targets‐TM perforation network diagram. HFO, Hippophae fructus oil; TM, tympanic membrane
FIGURE 3
FIGURE 3
GO (A) and KEGG (B) enrichment analysis. GO, gene ontology; HEGG, Kyoto Encyclopedia of Genes and Genomes
FIGURE 4
FIGURE 4
Protein–protein interactions diagram
FIGURE 5
FIGURE 5
Core proteins subnetwork diagram
FIGURE 6
FIGURE 6
Visualized results for molecular docking
See this image and copyright information in PMC

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