Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978 Apr;134(1):193-9.
doi: 10.1128/jb.134.1.193-199.1978.

Identification of a biochemically unique DNA-membrane interaction involving the Escherichia coli origin of replication

B L Craine, C S Rupert

Identification of a biochemically unique DNA-membrane interaction involving the Escherichia coli origin of replication

B L Craine et al. J Bacteriol. 1978 Apr.

Abstract

DNA-membrane complexes have been obtained from Escherichia coli by using a freeze-thaw lysis procedure that avoids lysozyme and detergents. Complexes made in this manner and containing DNA near the origin of replication are uniquely sensitive to ionic strength, Pronase, and trypsin. There is approximately one such complex per chromosomal origin. The sensitivities suggest that origin-specific binding is mediated by a protein. By using these unique characteristics to distinguish origin-specific complexes from the majority of DNA-membrane binding sites, it was found that the origin-specific binding persists after termination of chromosomal replication.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2720-4 - PubMed
    1. Nature. 1974 Sep 20;251(5472):252-4 - PubMed
    1. Genetics. 1974 Jun;77(2):199-220 - PubMed
    1. Bacteriol Rev. 1973 Dec;37(4):562-603 - PubMed
    1. J Bacteriol. 1973 Dec;116(3):1364-74 - PubMed

Publication types

MeSH terms

LinkOut - more resources