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. 2021 Nov 17:12:751552.
doi: 10.3389/fgene.2021.751552. eCollection 2021.

Genetic Diversity of Polymorphic Marker Merozoite Surface Protein 1 (Msp-1) and 2 (Msp-2) Genes of Plasmodium falciparum Isolates From Malaria Endemic Region of Pakistan

Shahid Niaz Khan  1 , Rehman Ali  1 , Sanaullah Khan  2 , Muhammad Rooman  3 , Sadia Norin  1 , Shehzad Zareen  1 , Ijaz Ali  4 , Sultan Ayaz  5
Affiliations

Genetic Diversity of Polymorphic Marker Merozoite Surface Protein 1 (Msp-1) and 2 (Msp-2) Genes of Plasmodium falciparum Isolates From Malaria Endemic Region of Pakistan

Shahid Niaz Khan et al. Front Genet. .

Abstract

Background: Understanding the genetic diversity of Plasmodium species through polymorphic studies can assist in designing more effective control strategies of malaria like new drug formulation and development of a vaccine. Pakistan is moderate endemic for Plasmodium falciparum, but little is known about the genetic diversity of this parasite. This study aimed to investigate the molecular diversity of P. falciparum based on msp-1 and msp-2 genes in the malaria-endemic regions of Khyber Pakhtunkhwa, Pakistan. Methods: A total of 199/723 blood samples, tested positive by microscopy for falciparum malaria, were collected from four districts (Dera Ismail Khan, Karak, Mardan, and Peshawar) of Khyber Pakhtunkhwa. Nested PCR amplification technique was employed to target block 2 of msp-1 and the central domain of msp-2 genes, including their respective allelic families K1, MAD20, RO33, FC27, and 3D7/IC, and to detect the extent of genetic diversity of P. falciparum clinical isolates. Results: Among the 199 microscopy-positive P. falciparum samples, a total of 192 were confirmed using PCR. Ninety-seven amplicons were observed for msp-1 and 95 for msp-2. A total of 33 genotypes, 17 for msp-1 (eight K1, six MAD20, and three RO33) and 16 for msp-2 (nine FC27 and seven 3D7/IC), were identified. The specific allelic frequency of the K1 family was higher (44.3%) than that of MAD20 (33.0%) and RO33 (23.0%) for msp-1, while the FC27 allelic family was dominant (60.0%) compared with 3D7/IC (40.0%) for msp-2. No polyclonal infection was observed in msp-1 and msp-2. The expected heterozygosity was 0.98 and 0.97 for msp-1 and msp-2, respectively. Conclusion: It was concluded that the P. falciparum populations are highly polymorphic, and diverse allelic variants of msp-1 and msp-2 are present in Khyber Pakhtunkhwa, Pakistan.

Keywords: Khyber Pakhtunkhwa; Pakistan; Plasmodium falciparum; genetic diversity; msp-1 and msp-2 genes; nested PCR; polymorphism.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Allelic families of msp-1 and msp-2 genes of Plasmodium falciparum 1 show the positive infection of Plasmodium falciparum; 2, 3, and 4 represent the corresponding alleles of msp-1; 5 and 6 show the alleles of msp-2; m is a 50-bp DNA ladder marker, and M is a 100-bp DNA ladder marker.
See this image and copyright information in PMC

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References

    1. A-Elbasit I. E., ElGhazali G., A-Elgadir T. M. E., Hamad A. A., Babiker H. A., Elbashir M. I., et al. (2007). Allelic Polymorphism of MSP2 Gene in Severe P. Falciparum Malaria in an Area of Low and Seasonal Transmission. Parasitol. Res. 102 (1), 29–34. 10.1007/s00436-007-0716-3 - DOI - PubMed
    1. Aubouy A., Migot-Nabias F., Deloron P. (2003). Polymorphism in Two Merozoite Surface Proteins of Plasmodium Falciparum Isolates from Gabon. Malar. J. 2, 12. 10.1186/1475-2875-2-12 - DOI - PMC - PubMed
    1. Babiker H. A., Charlwood J. D., Smith T., Walliker D. (1995). Gene Flow and Cross-Mating in Plasmodium Falciparum in Households in a Tanzanian Village. Parasitology 111 (Pt 4), 433–442. 10.1017/s0031182000065938 - DOI - PubMed
    1. Babiker H. A., Hill W. G., Lines J., Walliker D. (1997). Population Structure of Plasmodium Falciparum in Villages with Different Malaria Endemicity in East Africa. Am. J. Trop. Med. Hyg. 56 (2), 141–147. 10.4269/ajtmh.1997.56.141 - DOI - PubMed
    1. Cattamanchi A., Hubbard A., Kyabayinze D., Dorsey G., Rosenthal P. J. (2003). Distinguishing Recrudescence from Reinfection in a Longitudinal Antimalarial Drug Efficacy Study: Comparison of Results Based on Genotyping of Msp-1, Msp-2, and Glurp. Am. J. Trop. Med. Hyg. 68 (2), 133–139. 10.4269/ajtmh.2003.68.133 - DOI - PubMed