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. 2021 Nov 26:2021:5614861.
doi: 10.1155/2021/5614861. eCollection 2021.

miR-206 Inhibits Laryngeal Carcinoma Cell Multiplication, Migration, and Invasion

Yiling Liu  1 , YunTao Song  1 , Xiaojuan Chen  2 , Junfang Fan  3 , Wei Zheng  4 , Chen Cao  5
Affiliations

miR-206 Inhibits Laryngeal Carcinoma Cell Multiplication, Migration, and Invasion

Yiling Liu et al. J Healthc Eng. .

Retraction in

Abstract

Laryngeal carcinoma (LC) is one of the common human cancer types. MicroRNAs (miRNAs) were reported to be the essential regulators in cancer diagnosis, treatment, and prognosis. It was reported that miR-206 expression was reduced in various neoplastic diseases. However, the role and functional mechanism of miR-206 in LC progression remain unclear. In this research, miR-206 was found to be associated with tumor-node-metastasis (TNM) staging. In addition, the area under the curve (AUC) of miR-206 was 0.902 for diagnosis of LC and 0.854 for differential diagnosis of stage I-II and stage III-IV patients. Low expression of miR-206 was associated with poor prognosis of LC patients. miR-206 expression was an independent factor affecting the prognosis of LC patients, as revealed by the Cox regression analysis. In vitro experiments demonstrated that miR-206 overexpression reduced cell multiplication, invasion, and migration and increased cell apoptosis in LC cells. Moreover, SOX9 was a target of miR-206, and miR-206 negatively regulated SOX9 expression. Collectively, miR-206 might be a promising biomarker with diagnostic and prognostic value for LC, and the miR-206/SOX9 axis might be a candidate target for LC therapy.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
miR-206 was downregulated in tissues, serum, and cells of LC patients. (a) Measurement of miR-206 expression in tumor tissues and the adjacent normal tissues of LC patients by RT-qPCR. (b) Detection of miR-206 expression in serum of LC patients and healthy controls by RT-qPCR. (c) Correlation of miR-206 expression in serum and tissues of LC patients. (d) Determination of miR-206 expression in 16HBE, TR-LCC-1, and SNU899 cells by RT-qPCR. ∗P < 0.05.
Figure 2
Figure 2
Low expression of miR-206 was related to the poor prognosis of LC patients. (a) Serum miR-206 expression in LC patients with different TNM stages by RT-qPCR. (b) The ROC curve of serum miR-206 in diagnosis of LC patients. (c) The ROC curve of serum miR-206 in differentiating TNM staging. (d) Visualization of the overall survival of LC patients with high or low miR-206 expression. ∗P < 0.05.
Figure 3
Figure 3
miR-206 inhibited the multiplication, invasion, and migration and promoted cell apoptosis in LC cells. (a) RT-qPCR analysis of miR-206 expression after transfection of miR-206 mim or miR-206 inh. (b) Measurement of cell multiplication by CCK-8 assay. (c) Detection of cell apoptosis by flow cytometry. (d, e) Determination of cell migration and invasion by Transwell assay. ∗P < 0.05.
Figure 4
Figure 4
SOX9 was a direct target of miR-206. (a) Potential binding sites of miR-206 and SOX9. (b) Dual-luciferase reporter assay. (c) Western blot detection of SOX9 protein expression after transfection of miR-206 mim or miR-206 inh. (d) RT-qPCR analysis of SOX9 expression in LC. (e) The expression of miR-206 was negatively correlated with SOX9 in LC. ∗P < 0.05.
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References

    1. Zhang X., Wu N., Wang J., Li Z. LncRNA MEG3 inhibits cell proliferation and induces apoptosis in laryngeal cancer via miR‐23a/APAF‐1 axis. Journal of Cellular and Molecular Medicine . 2019;23(10):6708–6719. doi: 10.1111/jcmm.14549. - DOI - PMC - PubMed
    1. Yue P.-J., Sun Y.-Y., Li Y.-H., Xu Z.-M., Fu W.-N. MYCT1 inhibits the EMT and migration of laryngeal cancer cells via the SP1/miR-629-3p/ESRP2 pathway. Cellular Signalling . 2020;74 doi: 10.1016/j.cellsig.2020.109709.109709 - DOI - PubMed
    1. Zhao Q., Zheng X., Guo H., et al. Serum exosomal miR-941 as a promising oncogenic biomarker for laryngeal squamous cell carcinoma. Journal of Cancer . 2020;11(18):5329–5344. doi: 10.7150/jca.45394. - DOI - PMC - PubMed
    1. Franz L., Alessandrini L., Fasanaro E., et al. Prognostic impact of neutrophils-to-lymphocytes ratio (NLR), PD-L1 expression, and tumor immune microenvironment in laryngeal cancer. Annals of Diagnostic Pathology . 2021;50 doi: 10.1016/j.anndiagpath.2020.151657.151657 - DOI - PubMed
    1. Innocentini L. M. A. R., Teixeira A. H., Casemiro L. A., et al. Laryngeal cancer attributable factors and the influence on survival rates: a single Brazilian institution experience. International Archives of Otorhinolaryngology . 2019;23(3):299–304. doi: 10.1055/s-0039-1687909. - DOI - PMC - PubMed

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