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. 2021 Nov 12:8:703956.
doi: 10.3389/fvets.2021.703956. eCollection 2021.

Effects of Two Polysaccharides From Traditional Chinese Medicines on Rat Immune Function

Yu Lei Wang  1 , Xiang Hua Shu  1 , Xue Zhang  1 , Yong Bo Liu  2 , Ya Jing Zhang  1 , Tao Lv  1 , Xin Huang  1 , Chun Lian Song  1
Affiliations

Effects of Two Polysaccharides From Traditional Chinese Medicines on Rat Immune Function

Yu Lei Wang et al. Front Vet Sci. .

Abstract

The aim is to study the immune function effect of two polysaccharides extracted from traditional Chinese herbs on rats. Ultrasonic-assisted extraction was used to extract the polysaccharide from traditional Chinese medicines. MTT assay was used to determine the effects of two polysaccharides on the conversion of pig peripheral T lymphocytes. For this, 24 Sprague-Dawley rats were selected for the clinical trial and divided into groups B (blank), CK (cyclophosphamide inhibitory control), AP (angelica polysaccharide), and RIP (radix isatidis polysaccharide). Except for group B, other groups can induce the immunodeficiency by using cyclophosphamide. Rats of the AP and RIP groups were given gavage of 1 mL of AP and RIP. The blood was sampled from the eyeball on days 0, 7, 14, 21, 28, and 35, respectively, to determine immune cells, IgG and IgM of immunoglobulin, body weight, and spleen index. Results: The average content of AP and RIP was 51.27 and 14.8%, and the extraction rate was 75.23 and 60.94%. The maximum stimulation index was 1.407 when the concentration of AP was 8,000 μg mL-1 and 1.5 when the concentration of RIP was 125 μg mL-1. Both kinds of polysaccharides can alleviate the decline of white blood cells, lymphocytes, monocytes, neutrophils, and serum IgG and IgM caused by cyclophosphamide. The two polysaccharides can regulate the rapid recovery of weight in immunosuppressed rats and increase the spleen index of immunosuppressed SD rats. The polysaccharides from the two traditional Chinese medicines can alleviate the immunosuppression caused by cyclophosphamide and promote the immune function of the body, which can be used as raw material resources of new veterinary medicine.

Keywords: angelica; immune function; polysaccharides; radix isatidis; rats.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Experimental results of lymphocyte transformation from TCM polysaccharides.
Figure 2
Figure 2
Changes of WBC, Lym, Mon, and Gran.
Figure 3
Figure 3
Changes of immunoglobulin G (IgG) and IgM. ⋆ showed P < 0.05, meaning there is significance; ⋆⋆ showed P < 0.01, meaning there is extreme significance.
Figure 4
Figure 4
Comparison of the weight. ⋆ showed P < 0.05, meaning there is significance; ⋆⋆ showed P < 0.01, meaning there is extreme significance.
Figure 5
Figure 5
Comparison of the spleen index. ⋆ showed P < 0.05, meaning there is a significant difference compared with the RIP and CK groups; ⋆⋆ showed P < 0.01, meaning there is an extremely significant difference compared with the RIP and CK groups.
See this image and copyright information in PMC

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