Gamma radiation-induced synthesis of a novel chitosan/silver/Mn-Mg ferrite nanocomposite and its impact on cadmium accumulation and translocation in brassica plant growth

Abstract

Herein, a novel chitosan/silver/Mn_0.5Mg_0.5Fe₂O₄ (Cs/Ag/MnMgFe₂O₄) nanocomposite was synthesized with gamma irradiation assistant. The prepared Cs/Ag/MnMgFe₂O₄ nanocomposite was characterized via EDX, XRD, SEM, UV-vis spectroscopy. To evaluate the effects of soak low and high-dose nanocomposite on physiological parameters, photosynthetic pigments, antioxidant and non-antioxidant enzymes of cabbage under Cd stress, a factorial experiment was conducted based on CRD with five replications. The Cd stress decreased the morphological characteristics and photosynthetic pigments while increasing cabbage's antioxidant and non-antioxidant enzymes. The application of low and high-dose of nanocomposite decreased Cd content in leaves by about 42.86%, 60.48%, and the root by approximately 18.72%, 28.72%, respectively, and translocation factors and tolerance index, H₂O₂, O₂, and malondialdehyde. In contrast, the application of high of the nanocomposite increased the values of SPAD chlorophyll about 27.50%, stomatal conductance about 87.18%, net photosynthetic rate about 44.90%, intercellular CO₂ concentration about 32.00%, and transpiration rate about 85.20%, as compared to Cd stress. Furthermore, the application of low and high-dose Cs/Ag/MnMgFe₂O₄ nanocomposite enhances the antioxidant and non-antioxidant enzymes of the cabbage plant compared to Cd stress. Generally, it was conducted that Cs/Ag/MnMgFe₂O₄ nanocomposite can be used as a proper tool for increasing cabbage plants under Cd stress.

Keywords: Antioxidant; Cadmium translocation; Chitosan nanocomposites; Gamma radiation; Spinel ferrite.