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Review
. 2022 Feb:180:110032.
doi: 10.1016/j.apradiso.2021.110032. Epub 2021 Nov 18.

An automated radiosynthesis of [18F]DPA-714 on a commercially available radiosynthesizer, Elixys Flex/Chem

Katelyenn S McCauley  1 , Justin H Wilde  1 , Sophia M Bufalino  2 , Kiel D Neumann  3
Affiliations
Review

An automated radiosynthesis of [18F]DPA-714 on a commercially available radiosynthesizer, Elixys Flex/Chem

Katelyenn S McCauley et al. Appl Radiat Isot. 2022 Feb.

Abstract

[18F]DPA-714 is a radiotracer specific to the translocator protein (TSPO) and is useful for in vivo Positron Emission Tomography imaging studies. In this report, we have developed an automated radiosynthesis of [18F]DPA-714 on a commercially-available radiosynthesis platform, which comports with USP <823> guidelines. The wide availability of the radiosynthesis module and ease of dissemination of the production sequence will facilitate preclinical and clinical research of TSPO-related pathology.

Keywords: Automation; F-18; Fluorine-18; Positron emission tomography; Radiochemistry; Radiosynthesis.

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Conflict of interest statement

Declaration of competing interest

The authors declare no competing interests.

Figures

Figure 1.
Figure 1.
Reagents and consumables needed to complete the radiosynthesis of [18F]DPA-714 on the Elixys Flex/Chem™.
Figure 2
Figure 2. Influence of Time on Activity Yields of [18F]DPA-714.
All reactions were performed at 100 °C on the Elixys Flex/Chem module. All activity yields are reported as an average (n=3).
Figure 3
Figure 3. Influence of Temperature on Activity Yields of [18F]DPA-714.
All reactions were performed for 15 minutes on the Elixys Flex/Chem module. All activity yields are reported as an average (n=3).
Scheme 1.
Scheme 1.. Synthetic organic route to the radiochemical precursor of [18F]DPA-714.
The hypothesized formation of the elimination side product observed during the production of 8 is highlighted (lower right).
Scheme 2.
Scheme 2.
Radiochemical Synthesis of [18F]DPA-714.
See this image and copyright information in PMC

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