Gut microbiota disturbance exaggerates battery wastewater-induced hepatotoxicity through a gut-liver axis

Abstract

As the primary source of electricity for various devices, batteries are important contributors to the overall electronic waste generated; and are widely considered a source of highly ecotoxic pollutants. Material leakage in battery manufacturing has not been completely solved, and the elucidation of the toxic mechanisms of battery wastewater exposure is needed. We demonstrated that battery waste exposure disrupted the intestinal flora and aggravated hepatotoxicity via the gut-liver axis. Under battery waste exposure, colon epithelium suffered physiological damage, and gene and protein expression levels related to gut barrier function (ZO-1, claudin-1, and Occludin) were significantly downregulated. Meanwhile, battery waste reduced the richness and diversity of the flora, causing metabolites produced by intestinal microbes to enter the gut-liver axis. Gut microbial dysbiosis impaired mitochondrial respiratory function in liver tissue cells, and mitophagy, apoptosis, and the disorder of glycolipids and amino acid metabolism were induced in hosts exposed to battery toxins. Altogether, these results provided novel insights into the underlying mechanisms of battery wastewater-related hepatotoxicity induced by gut microbiota via the gut-liver axis, which has public health implications where humans and animals are exposed to industrial toxins generated by uncontained battery disposal.

Keywords: Battery wastewater; Gut microbiota; Gut-liver axis; Hepatotoxicity.