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. 2021 Nov 28:2021:4005327.
doi: 10.1155/2021/4005327. eCollection 2021.

Correlations of mRNA Levels among Efflux Transporters, Transcriptional Regulators, and Scaffold Proteins in Non-Small-Cell Lung Cancer

Xieyi Zhang  1 , Wangyang Liu  2 , Kazue Edaki  1 , Yuta Nakazawa  1 , Hiroki Kamioka  2 , Atsushi Fujita  3 , Ryoichi Onozato  3 , Misa Iijima  4 , Shigeru Tsuchida  5 , Takahiro Arai  6 , Yukiyoshi Fujita  6 , Kenta Mizoi  1 , Takuo Ogihara  1   2
Affiliations

Correlations of mRNA Levels among Efflux Transporters, Transcriptional Regulators, and Scaffold Proteins in Non-Small-Cell Lung Cancer

Xieyi Zhang et al. Can J Infect Dis Med Microbiol. .

Abstract

Multidrug resistance (MDR) due to enhanced drug efflux activity of tumor cells can severely impact the efficacy of antitumor therapies. We recently showed that increased activity of the efflux transporter P-glycoprotein (P-gp) associated with activation of Snail transcriptional regulators may be mediated mainly by moesin in lung cancer cells. Here, we aimed to systematically evaluate the relationships among mRNA expression levels of efflux transporters (P-gp, breast cancer resistance protein (BCRP), and multidrug resistance-associated protein 2 (MRP2)), scaffold proteins (ezrin (Ezr), radixin (Rdx), and moesin (Msn); ERM proteins), and SNAI family members (Snail, Slug, and Smac) in clinical lung cancer and noncancer samples. We found high correlations between relative (cancer/noncancer) mRNA expression levels of Snail and Msn, Msn and P-gp, Slug and MRP2, and Smuc and BCRP. These findings support our previous conclusion that Snail regulates P-gp activity via Msn and further suggest that Slug and Smuc may contribute to the functional regulation of MRP2 and BCRP, respectively, in lung cancer cells. This trial is registered with UMIN000023923.

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Conflict of interest statement

The authors declare they have no conflicts of interest.

Figures

Figure 1
Figure 1
Correlations (r values) among absolute and relative mRNA expression levels of SNAI family members, ERM proteins, and efflux transporters in clinical lung cancer tissue samples. Each value in the part below the oblique line shows the correlation of the absolute values of the parameters in the corresponding row and column, and those in the part above the oblique line show the correlation of the relative values of the parameters in the corresponding row and column. The values on the oblique line show the correlation between relative and absolute values (R/A) of the same parameter. The boldface underline indicates p < 0.05.
Figure 2
Figure 2
Correlations of relative mRNA expression levels of Snail and Msn, Msn and P-gp, Slug and MRP2, and Smuc and BCRP.
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