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. 2021 Nov 25:12:100170.
doi: 10.1016/j.fochx.2021.100170. eCollection 2021 Dec 30.

Edible pectin film added with peptides from jackfruit leaves obtained by high-hydrostatic pressure and pepsin hydrolysis

Irving Antonio Brion-Espinoza  1 , Maricarmen Iñiguez-Moreno  1 , Juan Arturo Ragazzo-Sánchez  1 , Julio César Barros-Castillo  1 , Carolina Calderón-Chiu  1 , Montserrat Calderón-Santoyo  1
Affiliations

Edible pectin film added with peptides from jackfruit leaves obtained by high-hydrostatic pressure and pepsin hydrolysis

Irving Antonio Brion-Espinoza et al. Food Chem X. .

Abstract

Jackfruit (Artocarpus heterophyllus Lam.) is an evergreen tree that produces a high waste of leaves. This study evaluated the obtention of peptides from jackfruit leaves using pancreatin and pepsin, their antifungal activity, and their effect on pectin films. The protein content was 7.64 ± 0.12 g/100 g of jackfruit fresh leaves. Pancreatin produced a higher yield than pepsin in the obtention of peptides (p ≤ 0.05). However, peptides obtained after 2 h by pepsin hydrolysis (Pep-P) had six essential amino acids and inhibited > 99% of mycelial growth and spore germination of Colletotrichum gloeosporioides. Pectin films with Pep-P showed a slight brown color, lower thickness, water vapor permeability, and moisture content, as well as higher thermal stability and better inhibition properties against C. gloeosporioides than pectin films without Pep-P (p ≤ 0.05). Pectin films added with Pep-P from jackfruit leaf could be a green alternative to anthracnose control in tropical fruits.

Keywords: Acetonitrile (PubChem CID: 6342 HPLC grade ≥99.8%); Amino acid profile; Amino acid standards (AA-S-18); Biocomposite film; Bradford reagent serine standard (PubChem CID: 5951); Colletotrichum gloeosporioides; Glycerol (PubChem CID: 753) sodium hydroxide (NaOH PubChem CID: 14798); Hydrochloric acid (HCl PubChem CID: 313); Jackfruit leaf; L-Norleucine (Nor ≥98% PubChem CID: 21236); Low-methoxyl (LM) pectin (29–33% Food grade E-440); N-tert-Butyldimethylsilyl-N-methyltrifluoroacetamide (MTBSTFA >97% PubChem CID: 2724275); Pancreatin (EC 232-468-9); Pepsin (EC 3.4.23.1); Postharvest protection.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig. 1
Fig. 1
Peptides from jackfruit leaves and their antifungal activity. Peptides with pepsin (A) and pancreatin (B). Growth of Colletotrichum gloeosporioides in potato dextrose agar (PDA) without (C) and with peptides (680 μg/mL) from leaf protein concentrate (LPC) of jackfruit after 2 h of exposition to pepsin (D). Spores germination of C. gloeosporioides in potato dextrose broth without (E) and with peptides at 340 (F) and 680 (G) μg/mL, respectively, after 8 h of incubation at 28 °C. Growth of C. gloeosporioides in PDA covered with pectin film without (H) and with peptides (680 μg/mL) from LPC of jackfruit after 2 h of exposition to pepsin (I). Tests in PDA were incubated for 7 days at 28 °C.
Fig. 2
Fig. 2
Thermal analyses of pectin films with and without peptides from jackfruit leaves. (A) Thermogravimetry (TG) curves of components of films (glycerol ---; calcium chloride ─; pectin ─; peptides from Jackfruit leaf protein concentrate obtained with pepsin (Pep-P, ---). (B) TG curves pectin films with (PFP, ─) and without (PF, ---) peptides from jackfruit leaves.
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