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. 2021 Nov:2021:7148-7151.
doi: 10.1109/EMBC46164.2021.9630176.

Fabrication of Highly Sensitive Pt-black Electrochemical Sensors for GABA Detection

Sung Sik Chu, Paul Marsh, Hung A Nguyen, Carolyn E Jones, Miranda M Lim, Hung Cao

Fabrication of Highly Sensitive Pt-black Electrochemical Sensors for GABA Detection

Sung Sik Chu et al. Annu Int Conf IEEE Eng Med Biol Soc. 2021 Nov.

Abstract

GABA (Gamma-aminobutyric acid) is the main inhibitory neurotransmitter in the central nervous system of mammals. It is known to be related with various neurological disorders. GABA plays a crucial role in normal neuronal activity, information processing and plasticity, and neuronal network synchronization. To date, microdialysis has been widely used to monitor the level of GABA but the temporal and spatial resolution is limited. Besides, electrochemical sensors for neurotransmitter measurement, having high temporal and spatial resolution, overcome this problem. Here, using a cost-effective method of electrodeposition of platinum black (Pt-black), a highly sensitive, GABA specific, amperometric electrochemical sensor is fabricated. Nanostructured Pt-black increases the active surface area of the electrode contributing to higher sensitivity. Along with that, a self-referencing site and an exclusion layer are integrated to increase the selectivity and the signal-to-noise ratio (SNR) of the biosensor. This provides a prototype for a highly sensitive GABA sensor that could later be used to study various neurological disorders related to GABA concentrations.Clinical Relevance- This electrochemical sensor allows real-time monitoring of major inhibitory neurotransmitter (GABA) with high sensitivity which can be used for studying various neurological disorders.

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Figures

Figure 1.
Figure 1.
Fabrication process of the biosensor.
Figure 2.
Figure 2.
(a) Cyclic voltammograms of bare Pt and Pt-black coated Pt electrodes in 5 mM of K3Fe(CN)6. Inset shows scanning electron microscopic images of bare Pt and Pt-black coated Pt electrodes (bar = 500 nm). (b) Comparison of current increase from GABA between bare Pt and Pt-black coated platinum electrode (n=5 each).
Figure 3.
Figure 3.
(a) Amperometric curve for Pt-black on platinum to sequential addition of GABA. (b) Corresponding calibration curve with sensitivity.
Figure 4.
Figure 4.
Selectivity test of Pt-black coated platinum electrode.
See this image and copyright information in PMC

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