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Comparative Study
. 1986 Sep;24(3):330-2.
doi: 10.1128/jcm.24.3.330-332.1986.

Rapid microprocedure for isolating detergent-insoluble outer membrane proteins from Haemophilus species

G M Carlone, M L Thomas, H S Rumschlag, F O Sottnek
Comparative Study

Rapid microprocedure for isolating detergent-insoluble outer membrane proteins from Haemophilus species

G M Carlone et al. J Clin Microbiol. 1986 Sep.

Abstract

A rapid microprocedure for isolating detergent (sodium N-lauroyl sarcosinate)-insoluble major outer membrane proteins from Haemophilus species produced results qualitatively identical to those obtained with a commonly used preparative isolation procedure. Proteins isolated by both procedures were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis after staining with Coomassie brilliant blue R-250. The time for outer membrane protein isolation was substantially reduced with the rapid procedure, allowing a larger number of membrane preparations to be obtained rapidly for routine analysis.

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References

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