Transcriptional identification of genes light-interacting in the extraretinal photoreceptors of the crayfish Procambarusclarkii

Abstract

Crayfish serve as a model for studying the effect of environmental lighting on locomotor activity and neuroendocrine functions. The effects of light on this organism are mediated differentially by retinal and extraretinal photoreceptors located in the cerebroid ganglion and the pleonal nerve cord. However, some molecular aspects of the phototransduction cascade in the pleonal extraretinal photoreceptors remain unknown. In this study, transcriptome data from the pleonal nerve cord of the crayfish Procambarusclarkii (Girard,1852) were analyzed to identify transcripts that potentially interact with phototransduction process. The Illumina MiSeq System and the pipeline Phylogenetically Informed Annotation (PIA) were employed, which places uncharacterized genes into pre-calculated phylogenies of gene families. Here, for the first time 62 transcripts identified from the pleonal nerve cord that are related to light-interacting pathways are reported; they can be classified into the following 11 sets: 1) retinoid pathway in vertebrates and invertebrates, 2) photoreceptor specification, 3) rhabdomeric phototransduction, 4) opsins 5) ciliary phototransduction, 6) melanin synthesis, 7) pterin synthesis, 8) ommochrome synthesis, 9) heme synthesis, 10) diurnal clock, and 11) crystallins. Moreover, this analysis comparing the sequences located on the pleonal nerve cord to eyestalk sequences reported in other studies reveals 94-100% similarity between the 55 common proteins identified. These results show that both retinal and pleonal non-visual photoreceptors in the crayfish equally expressed the transcripts involved in light detection. Moreover, they suggest that the genes related to ocular and extraocular light perception in the crayfish P.clarkii use biosynthesis pathways and phototransduction cascades commons.

Keywords: Caudal photoreceptor; opsins; photoresponse; phototransduction; pleonal nerve cord.

Figure. 1.

Comparative alignments of opsins reported in the crayfish ProcambarusclarkiiA long-wavelength-sensitive opsin (UniProtKB/Swiss-Prot: P35356.1; Hariyama et al. 1993); (GenBank: ALJ26467 1; Kingston and Cronin 2015); (Procl_ES_23_0; Manfrin et al. 2015) B short-wavelength-sensitive opsin (GenBank: ALJ26468.1; Kingston and Cronin 2015); (Procl_ES_11143_0; Manfrin et al. 2015)