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. 1986 Oct 20;207(1):142-4.
doi: 10.1016/0014-5793(86)80028-x.

Purification of the enhancing factor from mouse intestines

R Mulherkar, A Saraf, A Wagle, M G Deo
Free article

Purification of the enhancing factor from mouse intestines

R Mulherkar et al. FEBS Lett. .
Free article

Abstract

A unique polypeptide, called enhancing factor (EF), which enhances the binding of labeled epidermal growth factor (EGF) to cells, has been isolated. It has been purified to homogeneity from the acid-soluble proteins of mouse intestines. Earlier, EF was partially purified by two cycles of gel-permeation chromatography on Bio-Gel columns. We now report the final purification of EF on high-performance liquid chromatography (HPLC), using a reverse-phase column (mu Bondapak C18). The purity of the protein was confirmed when a single peak was obtained in HPLC. Also, a single protein band was obtained in SDS-PAGE. Purified EF has the same properties in vitro as those reported earlier for partially purified EF.

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