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. 1986 Oct;62(5):321-8.
doi: 10.1136/sti.62.5.321.

Immunoblot analysis of antigens associated with Haemophilus ducreyi using serum from immunised rabbits

J M Saunders, J D Folds

Immunoblot analysis of antigens associated with Haemophilus ducreyi using serum from immunised rabbits

J M Saunders et al. Genitourin Med. 1986 Oct.

Abstract

Sodium dodecyl sulphate polyacrylamide gel electrophoresis and immunoblotting were used to characterise isolates of Haemophilus ducreyi. Isolates of H ducreyi were heterogeneous in protein composition, but isolates from single outbreaks appeared similar both in protein profiles and antigenic analysis. Rabbits immunised with H ducreyi responded with a vigorous humoral immune response in which multiple antigenic polypeptides were detected. The most prominent antigens had molecular masses of 67, 42, 22.5, and 20 kilodaltons.

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