Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022:2400:253-261.
doi: 10.1007/978-1-0716-1835-6_24.

Detection of Tomato Spotted Wilt Virus (TSWV) Infection in Plants Using DAS-ELISA and Dot-ELISA

Shibo Gao  1 , Jianxiang Wu  2
Affiliations

Detection of Tomato Spotted Wilt Virus (TSWV) Infection in Plants Using DAS-ELISA and Dot-ELISA

Shibo Gao et al. Methods Mol Biol. 2022.

Abstract

Plant viruses cause severe damages to crop productions each year worldwide. To prevent the losses caused by plant viruses, it is necessary to develop specific and efficient diagnostic tools to detect viruses. Among the current virus detection techniques, serological detection methods are considered to be rapid, simple, sensitive, and high throughput. Therefore, serological detection methods such as double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), triple antibody sandwich ELISA (TAS-ELISA), antigen coated plate-ELISA (ACP-ELISA), Dot-ELISA and tissue print-ELISA as well as colloidal gold immunochromatographic strip are now wildly used to detect viruses in plants. In this chapter, we describe the DAS-ELISA and Dot-ELISA methods, and their applications in the detection of Tomato spotted wilt virus (TSWV) infection in plants. These two methods can be easily adapted for diagnosis of other plant viruses.

Keywords: DAS-ELISA; Dot-ELISA; Serological detection; Tomato spotted wilt virus.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Wu JX, Ni YQ, Liu H et al (2014) Monoclonal antibody-based serological assays and immunocapture-RT-PCR for detecting Rice dwarf virus in field rice plants and leafhopper vectors. J Virol Methods 195:134–140 - DOI
    1. Le DT, Netsu O, Uehara-Ichiki T et al (2010) Molecular detection of nine rice viruses by a reverse-transcription loop-mediated isothermal amplification assay. J Virol Methods 170:90–93 - DOI
    1. Wu JX, Ni YQ, Liu H et al (2013) Development and use of three monoclonal antibodies for the detection of Rice black-streaked dwarf virus in field plants and planthopper vectors. Virol J 10:114 - DOI
    1. Guo LQ, Wu JY, Chen R et al (2020) Monoclonal antibody-based serological detection of Rice stripe mosaic virus infection in rice or leafhoppers. Virol Sin 35:227–234 - DOI
    1. Zhang Y, Gao YL, He WQ et al (2020) Monoclonal antibody-based serological detection of Potato virus M in potato plants and tubers. J Integr Agr 19:1283–1291 - DOI

Publication types

LinkOut - more resources