Counts on viable Bacteroides fragilis: a modification of the microdroplet technique
- PMID: 349074
- PMCID: PMC2129807
- DOI: 10.1017/s0022172400024839
Counts on viable Bacteroides fragilis: a modification of the microdroplet technique
Abstract
A modification of Sharpe's microdroplet counting technique is presented which permits viable counting of Bacteroides fragilis. Lysed horse blood was added to 0.1 soy digest agar droplets and 2% was found to be the optimal concentration which combined adequate intradroplet colony size with acceptable visibility on the counting screen of the Colworth Droplette machine.
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