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. 2022 Apr;57(2):341-356.
doi: 10.1111/jre.12964. Epub 2021 Dec 15.

Chemomechanical preparation influences the microbial community and the levels of LPS, LTA and cytokines in combined endodontic-periodontal lesions: A clinical study

Brenda P F A Gomes  1 , Vanessa B Berber  1 , Ariane C S Marinho  1 , Lidiane M Louzada  1 , Rodrigo Arruda-Vasconcelos  1 , Maicon R Z Passini  1 , Erica M Lopes  1 , Vanessa G A Pecorari  1   2 , Tsute Chen  3 , Bruce J Paster  4
Affiliations

Chemomechanical preparation influences the microbial community and the levels of LPS, LTA and cytokines in combined endodontic-periodontal lesions: A clinical study

Brenda P F A Gomes et al. J Periodontal Res. 2022 Apr.

Abstract

Background: This study was conducted to compare the microbiomes, the levels of lipopolysaccharides (LPS), lipoteichoic acid (LTA), and cytokines (interleukin [IL]-1β and tumor necrosis factor-alpha [TNF-α]), before and after chemomechanical preparation (CMP) of the root canals (RC) and their associated periodontal pockets (PP) in teeth with combined EPL.

Materials: Samples were taken from 10 RC and PP, before and after CMP. The microbiomes (next-generation sequencing, V3-V4 hypervariable region of the 16S rRNA gene), microbiome diversity (bioinformatics analyses), LPS (limulus amebocyte lysate), LTA, IL-1β, and TNF-α (ELISA) were evaluated. A statistical analysis was performed with significance level set at 5%.

Results: The most abundant phyla in both sites were Firmicutes and Proteobacteria. Comparative studies of bacterial genera species revealed that some increased and others decreased after CMP at both sites. A 3% reduction in Gram-negative bacteria (RC) and a 4% increase in Gram-positive bacteria (PP) were detected. LPS levels were 4.4 times higher in PP than in the RC. LTA was detected in all samples investigated. Higher levels of IL-1β and TNF-α were detected in both sites at baseline. After CMP, LPS, LTA, IL-1β and TNF-α were reduced in both sites.

Conclusion: The microbial community in the RC and PP in teeth with combined EPL indicated a similarity between both sites. CMP effectively reduced the microbial load and the LPS levels from teeth with EPL, and consequently diminished the cytokine levels. The reduction in LTA levels in the RC and PP proved challenging.

Keywords: bacteria; cytokines; endodontic-periodontal lesions; endotoxins; lipoteichoic acid; next-generation sequencing.

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References

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