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. 2022 Jan;89(1):23-38.
doi: 10.1002/mrd.23549. Epub 2021 Dec 15.

Hemodynamic, endocrine, and gene expression mechanisms regulating equine ovarian follicular and cellular development

Aurea Wischral  1   2 , Marilia Pastorello  1 , Melba O Gastal  1 , Mohd A Beg  3 , Eduardo L Gastal  1
Affiliations

Hemodynamic, endocrine, and gene expression mechanisms regulating equine ovarian follicular and cellular development

Aurea Wischral et al. Mol Reprod Dev. 2022 Jan.

Abstract

Ovulatory follicle development and associated oocyte maturation involve complex coordinated molecular and cellular mechanisms not yet fully understood. This study addresses the relationships among follicle diameter, follicle wall blood flow, follicular-fluid factors, and gene expression for follicle growth, steroidogenesis, angiogenesis, and apoptosis in granulosa/cumulus cells and oocytes during different stages from the beginning of largest/ovulatory follicle to impending ovulation in mares. The most remarkable findings were (i) a positive association between follicle development, follicle blood flow, intrafollicular follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol, progesterone, and messenger RNA (mRNA) expression for FSHR and LHCGR in granulosa cells of the largest/ovulatory follicle; (ii) a plateau or decrease in follicle diameter and blood flow and granulosa cell mRNA for FSHR, LHCGR, IGF1R, VEGFR2, CYP19A1, and CASP3 at the preovulatory stage; (iii) higher StAR and BCL2 and lower CASP3 mRNA in granulosa cells at the time of impending ovulation; (iv) greater IGF1R mRNA for granulosa cells at the predeviation stage; and (v) lower FSHR, LHCGR, IGF1R, and VEGFR2 mRNA in cumulus cells and greater LHCGR and IGF1R mRNA in oocytes at the ovulatory stage. This study is a critical advance in the understanding of molecular mechanisms of follicle development and oocyte maturation and is expected to be vital for future studies targeting potential markers.

Keywords: dominant and ovulatory follicles; follicle wall blood flow; granulosa and cumulus cells; hormones and gene expression; oocyte.

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References

REFERENCES

    1. Acosta, T. J., Beg, M. A., & Ginther, O. J. (2004). Aberrant blood flow area and plasma gonadotropin concentrations during the development of dominant-sized transitional anovulatory follicles in mares. Biology of Reproduction, 71, 637-642.
    1. Adams, G. P., Singh, J., & Baerwald, A. R. (2012). Large animal models for the study of ovarian follicular dynamics in women. Theriogenology, 78, 1733-1748.
    1. Almadhidi, J., Seralini, G. E., Fresnel, J., Silberzahn, P., & Gaillard, J. L. (1995). Immunohistochemical localization of cytochrome P450 aromatase in equine gonads. Journal of Histochemistry & Cytochemistry, 43, 571-577.
    1. Amsterdam, A., Koch, Y., Lieberman, M. E., & Lindner, H. R. (1975). Distribution of binding sites for human chorionic gonadotropin in the preovulatory follicle of the rat. Journal of Cell Biology, 67, 894-900.
    1. Anteby, E. Y., Hurwitz, A., Korach, O., Revel, A., Simon, A., Finci-Yeheskel, & Laufer, N. (1996). Human follicular nitric oxide pathway: Relationship to follicular size, oestradiol concentrations and ovarian blood flow. Human Reproduction, 11, 1947-1951.

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