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. 2021 Dec 15;148(24):dev200343.
doi: 10.1242/dev.200343. Epub 2021 Dec 16.

The zebrafish issue: 25 years on

Mary C Mullins  1 , Joaquín Navajas Acedo  2 , Rashmi Priya  3 , Lilianna Solnica-Krezel  4 , Stephen W Wilson  5
Affiliations

The zebrafish issue: 25 years on

Mary C Mullins et al. Development. .

Abstract

In the 1990s, labs on both sides of the Atlantic performed the largest genetic mutagenesis screen at that time using an emerging model organism: the zebrafish. Led by Christiane Nüsslein-Volhard in Tübingen, Germany, and Wolfgang Driever in Boston, USA, these colossal screens culminated in 1996 with the publication of 37 articles in a special issue of Development, which remains the journal's largest issue to this day. To celebrate the anniversary of the zebrafish issue and reflect on the 25 years since its publication, five zebrafish researchers share what the issue means to them, how it has contributed to their career and its impact on the zebrafish community.

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Conflict of interest statement

Competing interests The authors declare no competing or financial interests.

Figures

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Slides at the Tuübingen lab. Left: top two shelves in the warm room closet contained F3 embryo dishes to be screened. Lower shelf contains retest crosses of previous mutants found. Right: Janni's printed schedule for the screen. Note the yellow tab ‘You are here’ midway on left.
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Anamniote Rohon-Beard somatosensory neurons. These neurons detect peripheral stimuli, such as pain, touch or temperature, using their elaborated sensory arborizations. The image depicts a clonally labeled neuron in a 5-day-old zebrafish larva using a neurog1:GFP-CAAX construct (a kind gift from Anand Chandrasekhar, Bond Life Sciences Center, University of Missouri, USA) color coded with a depth look-up table (LUT).
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Heart of a 3 days post fertilization zebrafish embryo. This myl7:MYL9-mScarlet; myl7:H2B-GFP transgenic zebrafish line labels cardiomyocyte cytoskeleton (myl7:MYL9-mScarlet, depth color-coded) and cardiomyocyte nuclei (myl7:H2B-GFP, gray).
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Phenotypic classes of gastrulation mutants found in the Boston screen. From Solnica-Krezel et al. (1996).
See this image and copyright information in PMC

References

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