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. 2021 Dec 16;16(12):e0261228.
doi: 10.1371/journal.pone.0261228. eCollection 2021.

Circulating miR-147b as a diagnostic marker for patients with bacterial sepsis and septic shock

Ngo Tat Trung  1   2   3 , Tran Thi Lien  2 , Vu Viet Sang  2   4 , Nghiem Xuan Hoan  2   3 , Nguyen Dang Manh  2   4 , Nguyen Sy Thau  2   4 , Dao Thanh Quyen  2   3 , Tran Thi Thu Hien  3 , Phan Quoc Hoan  3 , Mai Hong Bang  2   5 , Thirumalaisamy P Velavan  2   6 , Le Huu Song  2   4
Affiliations

Circulating miR-147b as a diagnostic marker for patients with bacterial sepsis and septic shock

Ngo Tat Trung et al. PLoS One. .

Abstract

Background: Early diagnosis, precise antimicrobial treatment and subsequent patient stratification can improve sepsis outcomes. Circulating biomarkers such as plasma microRNAs (miRNAs) have proven to be surrogates for diagnosis, severity and case management of infections. The expression of four selected miRNAs (miR-146-3p, miR-147b, miR-155 and miR-223) was validated for their prognostic and diagnostic potential in a clinically defined cohort of patients with sepsis and septic shock.

Methods: The expression of plasma miRNAs was quantified by quantitative PCR (qPCR) in patients with bacterial sepsis (n = 78), in patients with septic shock (n = 52) and in patients with dengue haemorrhagic fever (DHF; n = 69) and in healthy controls (n = 82).

Results: The expression of studied miRNA was significantly increased in patients with bacterial sepsis and septic shock. The plasma miR-147b was able to differentiate bacterial sepsis from non-sepsis and septic shock (AUC = 0.77 and 0.8, respectively, p≤ 0.05), while the combination of plasma miR-147b and procalcitonin (PCT) predicted septic shock (AUC = 0.86, p≤ 0.05).

Conclusions: The plasma miR-147b may be an useful biomarker independently or in combination with PCT to support clinical diagnosis of sepsis and equally prognosis of patients with septic shock.

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Conflict of interest statement

All authors declare no conflict of interests in this study.

Figures

Fig 1
Fig 1. Expression of selected microRNAs is significantly elevated in sepsis patients compared to controls.
Relative expression of individual miRNA (miR-146-3p, miR-147b, miR-155 and miR-223) in patients with and without sepsis. Non-sepsis group includes healthy controls (HC) and dengue hemorrhagic fever (DHF). P values were calculated by Wilcoxon tests.
Fig 2
Fig 2. High expression of selected microRNAs is associated with septic shock.
Relative expression of individual miRNA (miR-146-3p, miR-147b, miR-155 and miR-223) in sepsis patients with and without shock. P values were calculated by Wilcoxon tests.
Fig 3
Fig 3. The value of miRNAs’ in differentiating diagnosis bacterial sepsis and non-sepsis groups.
Diagnostic performance of the models built based on random forest models with single or double combination of miRNAs in differentiating sepsis patients from other groups HC, DHF and non-sepsis.
Fig 4
Fig 4. Combination of miR-147b and serological PCT can predict septic shock.
Diagnostic performance of the models built based on random forest models with single or double combination of miRNAs or double combination of individual miRNA and PCT in differentiating septic shock patients from non-septic shock patients.
See this image and copyright information in PMC

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