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. 2021 Dec 17;20(1):84.
doi: 10.1186/s12941-021-00488-x.

Epidemiological profile of multidrug-resistant and extensively drug-resistant Mycobacterium Tubrculosis among Congolese patients

Darrel Ornelle Elion Assiana  1   2 , Jabar Babatunde Pacôme Achimi Abdul  3 , Laure Stella Ghoma Linguissi  1   4 , Micheska Epola  3 , Jeannhey Christevy Vouvoungui  1   2 , Albert Mabiala  5 , Christopher Mebiame Biyogho  3 , Jean Ronald Edoa  3 , Bayodé Roméo Adegbite  3 , Ayola Akim Adegnika  3   6   7 , Linzy Elton  8 , Julio Ortiz Canseco  8 , Timothy D McHugh  8 , Gabriel Ahombo  2 , Francine Ntoumi  9   10   11
Affiliations

Epidemiological profile of multidrug-resistant and extensively drug-resistant Mycobacterium Tubrculosis among Congolese patients

Darrel Ornelle Elion Assiana et al. Ann Clin Microbiol Antimicrob. .

Abstract

Background: There is paucity of data on the prevalence and distribution of multidrug- Resistant-Tuberculosis (MDR-TB) in the Republic of Congo. Among the challenges resides the implementation of a robust TB resistance diagnostic program using molecular tools. In resource limited settings there is a need to gather data to enable prioritization of actions. The objective of this study was is to implement molecular tools as a best of diagnosing MDR and XDR-TB among presumptive tuberculosis patients referred to reference hospital of Makelekele in Brazzaville, Republic of the Congo.

Methods: We have conducted a cross-sectional study, including a total of 92 presumptive pulmonary tuberculosis patients and who had never received treatment recruited at the reference hospital of Makelekele from October 2018 to October 2019. The socio-demographic and clinical data were collected as well as sputum samples. Rifampicin resistance was investigated using Xpert (Cepheid) and second-line TB drugs Susceptibility testing were performed by the Brucker HAIN Line Probe Assay (GenoType MTBDRsl VER 2.0 assay) method.

Results: From the 92 recruited patients, 57 (62%) were found positive for the Mycobacterium tuberculosis complex. The prevalence of rifampicin-resistant tuberculosis (RR-TB) was 9.8% (9/92) and importantly 2.2% were pre-XDR/XDR.

Conclusion: This study showed a high rate of rifampicin resistance and the presence of extensively drug-resistant tuberculosis in the study area in new patients. This study highlights the need for further studies of TB drug resistance in the country.

Keywords: Epidemiological profile; Extensively drug-resistant TB; Line Probe Assay SL; Multidrug-resistant TB; Republic of Congo; Xpert MTB / RIF.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Flowchart of patient recruitment at Makelekele hospital and laboratory methods used
See this image and copyright information in PMC

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